【摘要】 为探究小豆复叶发育调控机理，本研究以电子束辐射小豆栽培品种京农6号获得的一个稳定遗传的五出复叶突变体pcl (pentafoliate compound leaf)为材料，进行了主要农艺性状调查、遗传分析、基因定位和转录组分析。结果表明，五出复叶小豆个体的株高、茎粗、单株荚数、荚宽、单荚粒数、单株产量和百粒重较三出复叶个体均显著增加。遗传分析结果表明，五出复叶由一对隐性基因控制。将突变基因定位于5号染色体末端上物理距离约为500 kb的区间内，区间内共注释出27个基因。染色体步行结果发现，VaTMK3基因启动子区域TATA-box中存在碱基突变，该突变位点分析与性状共分离。转录组和实时荧光定量PCR(qRT-PCR)分析结果显示，参与生长素信号转导和细胞扩增和增殖调控基因VaTMK3表达显著上调。同时生长素跨膜转运载体蛋白编码基因VaPIN1a、VaPIN1b、VaLAX1、VaLAX3、VaLAX5表达量均增加。由此初步确定五出复叶突变候选基因为VaTMK3，且VaTMK3影响了叶片中生长素的转运，从而影响了复叶发育。本研究为进一步解析小豆复叶发育调控分子机制提供了理论依据，为后续小豆复叶发育调控网路的建立奠定了研究基础。更多还原

【Abstract】 To explore the regulation mechanism of compound leaf development of Adzuki bean, a stable genetic pentafoliate compound leaf mutant pcl(pentafoliate compound leaf) was obtained from Adzuki bean cultivar jingnong 6 with electron beam irradiation. The main agronomic traits, genetic analysis, gene mapping, and transcriptome analysis were carried out. The results showed that plant height, stem diameter, the number of pods per plant and pod width, the number of pods per plant and yield per plant, and 100-seed weight of pentafoliate compound leaf adzuki bean were significantly higher than those of trifoliolate leaf adzuki bean individual. Genetic analysis showed that pentafoliate compound leaf were controlled by a pair of recessive genes. The mutant locus was located with a region of about 500 Kb on the end of chromosome 5, and a total of 27 genes were annotated in this region. A base mutation was found in the TATA-box of the promoter region of VaTMK3 gene by chromosome walking, and the mutation site was co-segregated with the trait.Transcriptome and real-time quantitative PCR(qRT-PCR) analysis showed that the expression of VaTMK3, which is involved in auxin signal transduction and regulation of cell expansion and proliferation, was significantly up-regulated. Meanwhile, the expression levels of auxin transmembrane transporter protein genes VaPIN1a, VaPIN1b, VaLAX1, VaLAX3, and VaLAX5 were increased. It was preliminarily determined that the candidate gene of pentafoliate leaf leaf mutations was VaTMK3, which affected the transport of auxin in leaves and thus affected the development of compound leaves. This study provided a theoretical basis for further analysing the molecular mechanism of the regulation of adzuki bean compound leaf development. It laid a foundation for the development regulation network of Adzuki bean compound leaves.更多还原