【摘要】 为研究常见绿藻对模拟酸雨胁迫的生理生化响应及其抗性，以1种浒苔、3种石莼为试验材料，设置5个pH水平，对其最大光化学量子产量（F_v/F_m）、相对电导率、可溶性蛋白和可溶性糖含量及抗氧化酶活性等生理指标进行测定。结果表明，模拟酸雨抑制了最大光化学量子产量，且抑制幅度随酸度和处理时间的增加而增大，pH值3.5的模拟酸雨处理30 min，石莼的F_v/F_m是浒苔的2.8倍以上；相对电导率与模拟酸雨的酸度及处理时间成正比，且发生不同程度的质壁分离；pH值4.5的模拟酸雨降低了藻体中叶绿素a、类胡萝卜素和可溶性糖含量，浒苔的降幅明显高于石莼，而酸雨处理下藻体中可溶性蛋白含量有所增加，且随着时间延长愈加显著；pH值4.5的模拟酸雨抑制了藻体中过氧化氢酶、超氧化物歧化酶活性，但提高了过氧化物酶活性，且处理时间与过氧化物酶活性呈正相关，与超氧化物歧化酶、过氧化氢酶活性呈负相关。研究认为pH值4.5能作为模拟酸雨胁迫的常见绿藻生理生化试验条件，4种绿藻对模拟酸雨具有响应差异。相比于浒苔，石莼对模拟酸雨具有更好的耐受性。本研究结果为潮间带大型海藻的生态分布特征及潜在的种群变化趋势研究提供了理论基础。更多还原

【Abstract】 To study the physiological and biochemical responses and resistance of common green seaweeds to simulated acid rain stress,the maximum photochemical quantum yield,relative conductivity,soluble protein and sugar contents,and antioxidant enzyme activity of one species of Enteromorpha and three species of Ulva were measured under five different pH levels.The results showed that the simulated acid rain inhibited the maximum photochemical quantum yield (F_v/F_m),and the inhibition amplitude increased with the increase of acidity and treatment time.The F_v/F_m of Ulva treated with simulated acid rain at pH 3.5 for 30 min was2.8 times higher than that of Enteromorpha.Relative conductivity was directly proportional to the acidity of simulated acid rain and the treatment time,and plasma wall separation occurred to varying degrees.Simulated acid rain at pH 4.5 reduced the contents of chlorophyll a,carotenoids and soluble sugar in the seaweed,and the reduction of Enteromorpha was significantly higher than that of Ulva.However,the soluble protein content in the seaweed increased under acid rain treatment,which was more significant with time extension.Simulated acid rain at pH 4.5 inhibited the activities of catalase and superoxide dismutase,but increased the activity of peroxidase of seaweeds,and the treatment time was positively correlated with the activity of peroxidase,but negatively correlated with the activities of superoxide dismutase and catalase.In summary,pH 4.5 can be used as physiological and biochemical test conditions for common green seaweeds to simulated acid rain stress,and four species of green seaweeds have different responses to simulated acid rain stress.Compared with Enteromorpha,Ulva has better tolerance to simulated acid rain.Our results provided a theoretical basis for understanding the ecological distribution characteristics and potential population changes of seaweeds in the intertidal zone.更多还原