【摘要】 目的 通过转录组学数据筛选在促性腺激素细胞肿瘤发生发展中差异表达的关键基因、通路及潜在的相互作用，为肿瘤治疗提供新靶点。方法 选取GEO数据库中编号GSE26966数据中正常垂体（n=9）和促性腺激素细胞肿瘤样本（n=10）为研究对象，利用GEO2R筛选出在促性腺激素细胞肿瘤和正常垂体组织之间的差异表达基因，通路富集分析筛选出差异基因所富集的关键分子通路，通过STRING数据库建立差异基因之间的互作网络，并探讨关键通路中分子所形成的互作网络。结果 通过对GSE26966数据进行分析，得出在促性腺激素细胞肿瘤和正常垂体组织间有1 193个差异表达基因，其中上调的基因为363个，下调的基因为830。这些差异基因主要富集在PI3K-Akt信号通路、跨膜受体蛋白激酶活性通路和跨膜受体蛋白酪氨酸激酶活性通路。PI3K-Akt信号通路中有46个分子富集，其中有42个低表达。蛋白互作网络分析显示IL6为互作关系最多的基因，富集在PI3K-Akt信号通路中。结论PI3K-Akt信号通路中基因低表达推测是由于蛋白被磷酸化而激活，导致通路中剩余基因表达水平降低。PI3K-Akt信号通路在肿瘤发生、发展中起关键作用，可为后续促性腺激素细胞肿瘤的基础研究和治疗提供新方向。更多还原

【Abstract】 Objective To explore the differential genes and pathways involved in the development and progression of gonadotroph PitNETs and their potential interactions,thereby providing new targets for the treatment of gonadotroph PitNETs.Methods The microarray data from GSE26966 was selected for our study(normal tissue=9,gonadotroph PitNETs=10),and the differentially expressed genes between the two group were analyzed by GEO2R in GEO database.Pathway enrichment analysis was performed to get significant pathways in gonadotroph PitNETs,and protein-protein interaction(PPI) by STRING were performed to further investigate the interactions network between differently expressed genes.Results Bioinformatics analysis between gonadotroph PitNETs and normal tissues revealed that 1 193 mRNAs were differently expressed,including 363 up-regulated and 830 down-regulated.Pathway analysis revealed that these genes were mainly involved in PI3K-Akt signaling pathway,transmembrane receptor protein kinase activity and transmembrane receptor protein tyrosine kinase activity.There were 46 genes enriched in the PI3K-Akt signaling pathway,among which 42 were down-regulated.PPI analysis reveal that IL6 was the top one genes with the most interactions among all genes,which enriched in PI3K-Akt signaling pathway.Conclusion The low expressed genes enriched in PI3K-Akt signaling pathway may because the proteins were phosphorylated and activated.PI3K-Akt signaling pathway plays a key role in regulating the development and progression of gonadotroph PitNETs,which will provide new target for the treatment of gonadotroph PitNETs.更多还原