【摘要】 为了了解福建省莆田地区猪源肺炎克雷伯菌(Klebsiella pneumoniae)的耐药情况，试验采集猪粪便拭子样品共340份，采用细菌分离培养与PCR方法进行细菌分离鉴定，通过微量肉汤稀释法检测分离菌株对9种抗菌药物的耐药性，并检测耐药基因和整合子的携带情况。结果表明：从粪便拭子中共分离鉴定出58株肺炎克雷伯菌，分离率为17.1%(58/340)。分离菌株对四环素的耐药率最高，为96.6%(56/58);对呋喃妥因、氯霉素和多黏菌素的耐药率分别为84.5%(49/58)、81.0%(47/58)和50.0%(29/58);对亚胺培南的耐药率最低，为5.2%(3/58)。分离菌株中耐药基因aadA1、tetA、phoQ、phoP和oqxA的检出率较高，均大于70.0%;从13株分离菌株中检测到Ⅰ类整合子，携带率为22.4%(13/58),主要携带的是氨基糖苷核苷酸转移酶(ANT)aadA22耐药基因盒，未测到Ⅱ类整合子。说明福建省莆田地区猪源肺炎克雷伯菌的耐药情况比较严重。更多还原

【Abstract】 In order to understand the drug resistance of pig-derived Klebsiella pneumoniae in Putian region, Fujian Province, in this experiment, a total of 340 pig fecal swab samples were collected; bacterial isolation and culture and PCR methods were used for the isolation and identification of bacteria. The resistance of isolated strains to nine antimicrobials was detected by microbroth dilution method, and the carrying of drug resistance genes and integrons was detected. The results showed that a total of 58 strains of Klebsiella pneumoniae were isolated from fecal swabs, with a separation rate of 17.1%(58/340). The isolated strains had the highest resistance rate to tetracycline, 96.6%(56/58); the resistance rates to nitrofurantoin, chloramphenicol and polymyxin were 84.5%(49/58), 81.0%(47/58) and 50.0%(29/58), respectively; the lowest resistance rate to imipenem was 5.2%(3/58). The detection rates of drug resistance genes aadA1, tetA, phoQ, phoP and oqxA in the isolated strains were high, all of which were greater than 70.0%. Class I integron was detected from 13 isolates, with a carrying rate of 22.4%(13/58), which mainly included aminoglycoside nucleotide transfer(ANT) aadA22 resistance gene cassette, and no class II integron was detected. The results suggested that the drug resistance of pig-derived Klebsiella pneumoniae in Putian region, Fujian Province was serious.更多还原