【摘要】 目的 研究细颗粒物(PM_2.5)对人脐静脉内皮细胞（HUVEC）毒性及对活性氧（ROS）、丙二醛（MDA）、乳酸脱氢酶（LDH）水平影响，为PM_2.5毒性机制的研究提供参考数据。方法 采用体外培养的HUVEC细胞为研究对象，对生长状况良好的细胞给予（25、50、100、200、300μg/ml）浓度的PM_2.5染毒，MTT法检测细胞存活率，化学荧光法、硫代巴比妥酸（TBA）、微板法分别检测ROS、MDA、LDH等毒性指标变化，检查细胞凋亡率。结果 PM_2.5染毒6、12、18、24、36 h随着染毒剂量升高，细胞存活率均逐渐降低，同一染毒浓度随着染毒时间延长，细胞存活率同样下降（P<0.05）,且在染毒36 h, PM_2.5浓度为400μg/ml,细胞存活率仅为45.23%;不同浓度PM_2.5染毒HUVEC 24 h后，MDA、LDH、ROS水平，均随着染毒剂量增加而升高，并且300、400μg/ml达到最大值，差异有统计学意义（P<0.05）;经过PM_2.5染毒后细胞早期凋亡率、中晚期凋亡率以及死亡细胞率均随着染毒浓度升高具有上升趋势，其中早期凋亡率变化最明显，差异有统计学意义（P<0.05）。结论 PM_2.5对HUVEC毒性损伤表现明显，降低细胞存活率，提高细胞凋亡率。更多还原

【Abstract】 Objective To study the toxicity of fine particulates with 2.5 microns or less in diameter(PM2.5) exposure to human umbilical vein endothelial cells(HUVECs) and the effects on the levels of reactive oxygen species(ROS), malondialdehyde(MDA) and lactate dehydrogenase(LDH), so as to provide reference data for analyzing the toxicity mechanism of PM2.5.Methods In vitro cultured HUVECs in a good growth condition were exposed to 25, 50, 100, 200, and 300μg/ml PM2.5. The cell survival rate was measured by MTT assay. Changes in ROS, MDA, LDH and other toxic indicators were detected by chemical fluorescence, thiobarbituric acid(TBA), and microplate method, respectively. The apoptotic rate was measured.Results The cell survival rate gradually decreased with the increased dose of PM2.5 exposure for 6, 12, 18, 24 and 36 hours, which also decreased with the prolongation of exposure at an indicated dose of PM2.5(P<0.05). The cell survival rate was 45.23% with 400μg/ml of PM2.5 exposure for 36 hours. After 24 hours of PM2.5 exposure to HUVECs at different doses, MDA, LDH and ROS levels significantly increased with the increase in the exposed dose, and reached the maximum at 300 and 400μg/ml(P<0.05). After PM2.5 exposure, the rate of early, intermediate and late-stage apoptosis, and the rate of dead cells all showed an upward trend with the increase in the exposed dose, which was significantly pronounced in that of early apoptosis(P<0.05).Conclusion PM2.5 exposure has an obvious toxicity to HUVECs, with decreased survival rate and increased apoptotic rate.更多还原