【摘要】 12-氧-植物二烯酸还原酶(OPR)为黄素单核苷酸(FMN)依赖的氧化还原酶，是合成茉莉酸的关键酶，其对植物生长发育及防御调控具有重要作用。为研究OPR基因在玉米中的抗病作用，利用生物信息学方法，在31个玉米不同自交系中对OPR家族成员进行鉴定，并分析受玉米大斑病菌侵染后的表达规律。结果表明，在玉米B73品系中鉴定出了8个OPR基因，这些基因不均匀地分布于7条染色体上，且其表达蛋白富含酸性氨基酸。进一步分析发现，玉米OPR家族只有1种结构域Oxidored＿FMN,并且所有成员均包含已鉴定的10个蛋白质保守基序。利用MEGA软件对玉米、小麦、水稻和拟南芥的OPR家族成员进行系统发育关系分析，发现这些植物OPR基因家族进化关系具有明显差异，其中玉米和水稻的进化关系最为接近。运用OrthoFinder软件对其同源组分析发现，玉米OPR基因家族具有高度保守性，所有OPR基因均为核心基因，但其功能略有差别。进一步利用河北省植物生理与分子病理学重点实验室前期的RNA-seq数据，对玉米B73品系OPR基因响应大斑病菌侵染的表达模式进行分析，并通过qRT-PCR对基因表达模式进行验证，发现这些基因在病菌侵染玉米过程中呈现3种不同的表达模式。本研究系统的鉴定了玉米泛基因组OPR家族基因，以及确定了其在应对玉米大斑病菌侵染后的表达模式。更多还原

【Abstract】 12-oxo-phytodienoic(OPR) acid reductase is a flavin mononucleotide-dependent oxidoreductase, a key enzyme for the synthesis of jasmonic acid, which is important for plant growth, development and defence regulation.In order to study the disease resistance effect of OPR gene in maize, bioinformatics methods were used to identify OPR family members in 31 different maize inbred lines and the expression pattern after infestation by S. turcica was analyzed.The results showed that eight OPR genes were identified in maize line B73 and they were unevenly distributed on seven chromosomes.In addition, these expressed proteins of OPR genes were rich in acidic amino acids.Further analysis revealed that the maize OPR family contained only one structural domain, Oxidored＿FMN,and all OPR members contained 10 protein conserved motifs identified.Phylogenetic relationship analysis of OPR family members from maize, wheat, rice and Arabidopsis using MEGA software revealed significant differences in the evolutionary relationships of OPR gene families in these plants.The OPR proteins of maize and those of rice were the closest in evolution.Homology analysis using OrthoFinder software revealed that the maize OPR gene family was highly conserved, with all OPR genes being core genes, but with slightly different functions.According to the previous RNA-seq data of Hebei key Laboratory of Plant Physiology and Molecular Pathology, we analyzed the expression pattern of OPR genes of maize B73 in response to S.turcica infection and verified the gene expression pattern of OPR genes through qRT-PCR.It was found that there were three different expression patterns in the process of S.turcica infection.This study systematically identified the maize pan-genomic OPR family genes and their expression patterns in response to S.turcica infection.更多还原