【摘要】 为了探明碱胁迫对杂交鲟鲟龙1号皮肤生理功能的影响，采用转录组测序的方法和皮肤组织学切片研究观察了碱胁迫与正常养殖水质条件下杂交鲟皮肤组织的分子特征和组织结构。结果发现，对照组与胁迫组差异表达基因总数量(DEGs)为1 849个，其中1 302个基因在胁迫组的皮肤组织中上调，547个基因下调。对差异表达基因进行GO聚类，主要富集在胞浆钙离子浓度的调节、肌肉收缩、肌钙蛋白复合物、肌钙蛋白T结合、肌钙蛋白C结合等7个GO term上。KEGG富集分析发现，甘氨酸、丝氨酸和苏氨酸代谢，蛋白质的消化和吸收，淀粉和蔗糖代谢等为显著富集通路。代谢通路互作网络分析发现，碱胁迫下鲟龙1号皮肤核心代谢途径为黏着斑、蛋白质消化吸收和血小板活化、补体和凝血级联及缺氧诱导因子-1信号通路等途径为主效途径。组织切片结果显示，杂交鲟在碱胁迫下皮肤组织和肾脏组织受到严重损伤，肾小球和肾小管发生明显皱缩，远曲小管萎缩尤为明显，肾小球上皮细胞体积减小，管壁变薄甚至脱落；皮肤组织的黏液细胞数量随碱度的升高而增多，棒状细胞细胞核发生固缩现象，在碱胁迫下排列更加紧密。揭示了鲟龙1号皮肤组织碱胁迫响应相关基因的总体表达特征，同时可为鲟鱼盐碱驯养提供参考。更多还原

【Abstract】 To investigate the effect of alkaline stress on the skin physiology of hybrid sturgeon(Huso dauricus♀×Acipenser schrenckii♂),we investigated the molecular characteristics of the skin tissue of hybrid sturgeon under alkaline stress and normal breeding water conditions by transcriptome sequencing.The total number of differentially expressed genes(DEGs)between control and stress groups was the result showed that was 1 849,of which 1 302 genes were up-regulated genes and 547 genes were down-regulated in the skin tissue of the stress group.GO clustering of differentially expressed genes showed that they were mainly enriched in seven GO terms, including regulation of cytoplasmic calcium ion concentration, muscle contraction, troponin complex, troponin T binding, and troponin C binding.KEGG enrichment analysis revealed that glycine, serine and threonine metabolism, protein digestion and absorption, starch and sucrose metabolism were significantly enriched pathways.Interaction network analysis of metabolic pathways revealed that the core metabolic pathways of hybrid sturgeon skin under alkaline stress were the adherent patch, protein digestion and absorption and platelet activation, complement and coagulation cascade and hypoxia-inducible factor-1 signaling pathways as the main efficient pathways.Interaction network analysis of metabolic pathways indicated that the core metabolic pathways of hybrid sturgeon skin under alkaline stress were the adherent patch, protein digestion and absorption and platelet activation, complement and coagulation cascade and hypoxia-inducible factor-1 signaling pathways as the main efficient pathways.The results of histological sections showed that the skin and kidney tissues of hybrid sturgeon were severely damaged by alkaline stress, and the glomeruli and tubules were significantly wrinkled, especially the atrophy of the distal tubules, and the epithelial cells of the glomeruli were reduced in size, and the walls of the tubules were thinned or even detached; the number of mucous cells in the skin tissues increased with the increase of alkalinity, and the nuclei of rod-shaped cells were solidified and arranged more closely under alkaline stress.更多还原