【摘要】 建立了HPLC法同时测定R-四氢罂粟碱中的8个有关物质。根据化合物结构，选择C₁₈填料色谱柱，并依据药典记载对色谱柱及色谱条件进行筛选。结果表明：采用Kromasil 100-5 C₁₈（4.6 mm×250 mm,5μm）色谱柱，以10.0 g/L的甲酸铵-乙腈（100∶5）为流动相A，甲醇为流动相B，梯度洗脱；柱温37℃，检测波长280nm，流速为1.0mL/min，进样量10μL，可同时测定R-四氢罂粟碱中的8个有关物质，分离度均大于1.5；各化合物在0.5～10.0μg/mL范围内，相关系数R²≥0.9999，线性良好，重现性强，灵敏度高，可用于R-四氢罂粟碱中有关物质的检测。更多还原

【Abstract】 HPLC method was established for simultaneous determination of 8 related substances in R-tetrahydropapaverine. The C₁₈ packing column was selected according to the compound structure, and the column and chromatographic conditions were screened according to the pharmacopoeia. The results showed that Kromasil 100-5 C₁₈（4.6mm×250mm, 5μm） column was used with 10 g/L ammonium formate-acetonitrile（100∶5） as mobile phase A and methanol as mobile phase B. The column temperature was 37℃, the detection wavelength was 280nm, the flow rate was 1.0 mL/min, and the sample size was 10μL. The results showed that the separation degree of 8 related substances in R-tetrahydropapaverine was more than 1.5. The compounds were in the range of 0.5～10.0 μg/mL, the correlation coefficient R²≥0.9999, good linearity, strong reproducibility, high sensitivity, and can be used for the detection of related substances in R-tetrahydropapaverine.更多还原