【摘要】 目的 研究甘草酸苷对癫痫持续状态（SE）大鼠模型炎性因子释放的调节作用。方法 采用随机数表法将大鼠随机分为5组，每组6只，采用腹腔注射氯化锂和匹罗卡品法制作SE大鼠模型，根据腹腔内注射甘草酸苷剂量不同分别为：对照组、SE组、低剂量组、中剂量组和高剂量组，腹腔内注入不同剂量的甘草酸苷（20、40和60 mg/kg）。酶联免疫吸附试验（ELISA）检测SE大鼠模型血清中高迁徙率组蛋白1(HMGB1)、肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)和白介素6(IL-6)的表达水平。定量实时PCR(qRT-PCR)法检测海马组织中HMGB1和RAGE mRNA表达水平。结果 甘草酸苷可以有效减少SE发作后血清和海马组织中HMGB1、TNF-α、IL-1β和IL-6的释放水平，在注射甘草酸苷6 h后，与SE组比较，低剂量组和中剂量组HMGB1和炎性因子表达水平均有不同程度的下调，但差异无统计学意义（P> 0.05）；而高剂量组各项指标的下调差异有统计学意义（P <0.05），提示早期甘草酸苷的作用与用药剂量相关。注射甘草酸苷12、24 h后，与SE组比较，低剂量、中剂量和高剂量组血清中HMGB1和炎性因子的表达水平显著降低，差异有统计学意义（P <0.05），提示甘草酸苷对SE大鼠血清中HMGB1和炎性细胞因子水平的调节还与用药时间有一定关系。结论 甘草酸苷可以通过抑制HMGB1的表达从而减轻SE大鼠模型中炎性因子的升高。更多还原

【Abstract】 Objective To investigate the regulatory effect of glycyrrhizin on the release of inflammatory factors in a rat model of status epilepticus(SE). Methods Rats were randomly divided into 5 groups using a random number table method, with 6 rats in each group. A rat model of status epilepticus was established by intraperitoneal injection of lithium chloride and pilocarpine. The rats were divided into the control group, SE group, low-dose group, medium-dose group, and high-dose group according to the different doses of intraperitoneal injection of glycyrrhizin. Intraperitoneal injections of different doses of glycyrrhizin(20, 40 and 60 mg/kg) were conducted. Enzyme-linked immunosorbent assay(ELISA) was used to test the expression level of the high mobility group box 1(HMGB1) and tumor necrosis factor-α(TNF-α), interleukin-1 β(IL-1 β) and interleukin-6(IL-6) in the serum of SE rat model. Quantitative real-time PCR(qRT-PCR) was used to test the expression levels of HMGB1 and RAGE mRNA in the hippocampus.Results Glycyrrhizin could effectively reduce the release levels of HMGB1, TNF-α, IL-1 β and IL-6 in serum and hippocampus after SE onset. After 6 hours of injection of glycyrrhizin, the expression levels of HMGB1 and inflammatory factors in the low-dose and medium-dose groups were downregulated to varying degrees compared to the SE group, but the difference was not statistically significant(P > 0.05); The downregulation differences of various indicators in the high-dose group were statistically significant(P < 0.05), indicating that the effect of early glycyrrhizin was related to the dosage of medication. After 12 and 24 hours of injection of glycyrrhizin, compared with the SE group, the expression levels of HMGB1 and inflammatory factors in the serum of low-dose, medium-dose, and high-dose groups were significantly reduced, with a statistically significant difference(P < 0.05), which indicated that the regulation of glycyrrhizin on the levels of HMGB1 and inflammatory cytokines in the serum of SE rats was also related to the duration of medication. Conclusion Glycyrrhizin can alleviate the elevation of inflammatory factors in SE rat models by inhibiting the expression of HMGB1.更多还原