【摘要】 目的 研究华蟾素腹腔灌注对大鼠腹腔肠粘连的影响并初步探讨其抗炎机制。方法 取雄性SD大鼠40只，随机分为4组：假手术组、对照组、透明质酸钠组、华蟾素组，每组各10只。除假手术组外，其余3组大鼠均制备肠粘连模型。假手术组、对照组腹腔注射生理盐水5 ml/kg；透明质酸钠组和华蟾素组分别腹腔注射透明质酸钠5 ml/kg和华蟾素5 ml/kg。于术后第8天收集腹水0.2 ml，眼眶取血0.5 ml，酶联免疫吸附剂测定法检测腹水转化生长因子-β1浓度和静脉血肿瘤坏死因子-α含量。肉眼观察肠粘连的分级程度，并做病理切片观察盲肠组织与腹壁组织炎症程度和粘连程度。结果 华蟾素组的粘连程度明显低于对照组，差异有统计学意义（P <0.05）。华蟾素组间质中炎性细胞反应和纤维组织增生程度明显优于对照组，华蟾素组腹水转化生长因子-β1和静脉血肿瘤坏死因子-α的含量低于对照组，差异有统计学意义（P <0.05）。结论 华蟾素腹腔灌注可以抑制急性炎症期的炎细胞反应和抑制纤维组织增生，从而减轻腹腔肠粘连程度，其机制可能与抑制转化生长因子-β1和肿瘤坏死因子-α的过度表达有关。更多还原

【Abstract】 Objective To study the effect of intraperitoneal perfusion of cinobufotalin on abdominal intestinal adhesions in rats and to explore its anti-inflammatory mechanism. Methods A total of 40 male SD rats were randomly divided into four groups: the sham operation group, the control group, the sodium hyaluronate group and the cinobufotalin group, with 10 rats in each group. Except the sham operation group, intestinal adhesion model was established for the other three groups of rats. The sham operation group and the control group were intraperitoneally injected with normal saline at 5 ml/kg; the sodium hyaluronate group and the cinobufotalin group were intraperitoneally injected with sodium hyaluronate at 5 ml/kg and cinobufotalin at 5 ml/kg respectively. 0.2 ml of ascites and 0.5 ml of blood from the orbit were collected on the 8th day after operation. Enzyme-linked immunosorbent assay was utilized to detect the concentration of transforming growth factor-β1 in ascites and the content of tumor necrosis factor-α in venous blood. The grading degree of intestinal adhesion was observed with naked eyes, and pathological sections were prepared to observe the inflammatory degree and adhesion degree of cecum tissue and abdominal wall tissue. Results The adhesion degree of the cinobufotalin group was significantly lower than that of the control group, with statistically significant difference(P < 0.05). The inflammatory cell reaction and fibrous tissue proliferation degree in the interstitial tissue of the cinobufotalin group were significantly better than those of the control group. The contents of transforming growth factor-β1 in ascites and tumor necrosis factor-α in venous blood in the cinobufotalin group were lower than those of the control group, with statistically significant differences(P < 0.05). Conclusion Intraperitoneal perfusion of cinobufotalin can inhibit inflammatory cell reaction and fibrous tissue proliferation in the acute inflammatory phase,thereby reducing the degree of abdominal intestinal adhesion, and its mechanism may be related to the inhibited overexpression of transforming growth factor-β1 and tumor necrosis factor-α.更多还原