【摘要】 目的：探讨1-磷酸鞘氨醇-2型受体（S1PR2）在大鼠慢性根尖周炎病变过程中的表达变化。方法：选取20只SD大鼠，随机处死4只作为0 d观察对象；在其余大鼠的下颌第一磨牙开髓建立根尖周炎模型，并在7 d、14 d、21 d和28 d随机处死4只；大鼠根尖组织行苏木精—伊红（HE）染色，抗酒石酸酸性磷酸酶（TRAP）染色检测破骨细胞；实时荧光定量聚合酶链式反应（RTqPCR）检测S1PR2、核因子-κB受体活化因子配体（RANKL）、骨保护素（OPG）的表达量。结果：HE染色显示：0 d根尖未见明显异常，7 d少量炎细胞浸润，14 d炎细胞浸润增多，21 d骨组织溶解增加，28 d炎症细胞浸润明显。RT-qPCR结果显示：0～28 d S1PR2的m RNA表达量逐渐升高，7 d、14 d、21 d和28 d均高于0 d(P<0.05),RANKL表达量在0～21 d逐渐增加，21 d达到最高峰，28 d时降低，7 d、14 d、21 d和28 d均高于0 d(P<0.05);OPG表达量在0～14 d逐渐降低，14 d时达到最低值，14～28 d又升高，0 d、7 d和28 d均高于14 d(P<0.05)。TRAP染色结果显示：破骨细胞与RANKL表达趋势一致。S1PR2与RANKL表达量、破骨细胞数呈正相关关系（P<0.05）,RANKL与OPG表达量呈负相关关系（P<0.05）。结论：S1PR2的mRNA表达量随根尖周炎的发展而升高，提示其可能参与了慢性根尖周炎的发生、发展。更多还原

【Abstract】 Objective: To investigate the expression and changes of sphingosine-1-phosphate receptor 2(S1PR2)in chronic apical periodontitis of rats. Methods: 20 SD rats were selected, and 4 rats were sacrificed randomly as observation objects on 0 day. The model of apical periodontitis was established in the mandibular first molars of the other rats, and 4 rats were sacrificed randomly on day 7, 14, 21 and 28. HE staining and tartrate-resistant acid phosphatase(TRAP) were used to detect osteoclasts. The expressions of S1PR2, receptor activator of nuclear factor-κB ligand(RANKL) and osteoprotegerin(OPG) were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). Results: HE staining showed that no obvious abnormality was observed in root tips on day 0. A small amount of inflammatory cells infiltrated on day 7 and increased on day 14; osteolysis increased on day 21; inflammatory cell infiltration was obvious on day 28. RT-q PCR results showed that the mRNA expression of S1PR2 increased gradually from 0 d to 28 d, and was higher on day 7, 14, 21 and 28 than that on day 0(P<0.05). The expression of RANKL increased gradually from 0 to 21 d, reached its peak on day21, decreased on day 28, and was higher on day 7, 14, 21 and 28 than that on day 0(P<0.05). The expression of OPG decreased gradually from 0 d to 14 d, reached the lowest value on day 14, increased from 14 d to 28 d, and was higher on day 0, 7 and 28 than that on day 14(P<0.05). TRAP stainning results showed that the expression trend of osteoclasts and RANKL was consistent. S1PR2 was positively correlated with the expression of RANKL and osteoclast number(P<0.05), while RANKL expression was negatively correlated with OPG expression(P<0.05). Conclusion: S1PR2 mRNA expression increases with the development of apical periodontitis, suggesting that S1PR2 may be involved in the occurrence and development of chronic apical periodontitis.更多还原