【摘要】 为获得对八角叶甲（Oides duporti）具有高致病力的白僵菌（Beauveria spp.）菌株，为利用白僵菌防治八角叶甲提供理论依据，采用组织分离法从感病的八角叶甲幼虫体内分离获得5株白僵菌菌株，测定其菌落直径、产孢量和孢子萌发率，采用浸虫法测定5个菌株对八角叶甲的毒力，并结合形态学与分子生物学，对菌株进行鉴定。结果表明，菌落直径最大的为菌株YJ-43；菌株YJ-22的产孢量和孢子萌发率均最高，毒力最强。形态学及rDNA-ITS序列分析结果显示，5个菌株均为球孢白僵菌（B. bassiana）。更多还原

【Abstract】 In order to obtain strains of Beauveria spp. with high pathogenicity to Oides duporti and provide theoretical basis for controlling O. duporti with Beauveria spp., five strains of Beauveria spp. were isolated from infected O. duporti larvae by tissue isolation method, colony diameters, spore productions and spore germination rates of which were studied. Virulence of 5 strains against O. duporti were determined by immersion method. 5strains were identified by combination of morphology and molecular biology. Results showed that strain YJ-43had the largest colony diameters, and strain YJ-22 had the highest spore production and spore germination rate with the strongest virulence. 5 strains were identified as B. bassiana through morphology and rDNA-ITS sequence analysis.更多还原