【摘要】 目的 探讨长链非编码RNA（lncRNA）FIRRE对过氧化氢（H₂O₂）诱导的心肌细胞损伤的影响。方法 将H9c2细胞分别转染si-lncRNA FIRRE、miR-490-3p mimics或共转染si-lncRNA FIRRE与anti-miR-490-3p,然后采用200μmol/L H₂O₂处理24 h,实时荧光定量PCR（RT-qPCR）法检测细胞lncRNA FIRRE和miR-490-3p的相对表达量，CCK-8检测细胞增殖抑制率，流式细胞术检测细胞凋亡，试剂盒检测细胞丙二醛（MDA）、氢酶（LDH）水平及超氧化物歧化酶（SOD）活性，双荧光素酶报告实验验证lncRNA FIRRE和miR-490-3p的靶向关系，免疫印迹法检测裂解的半胱氨酸天冬氨酸蛋白酶-3（Cleaved-caspase-3）的蛋白表达水平。结果 经200μmol/L H₂O₂处理24 h后，H9c2细胞lncRNA FIRRE相对表达量升高（P<0.05）,细胞增殖抑制率、凋亡率、Cleaved-caspase-3蛋白水平、细胞培养上清液中LDH及MDA水平均升高（P<0.05）,miR-490-3p水平、SOD活性降低（P<0.05）。沉默lncRNA FIRRE或过表达miR-490-3p可降低细胞增殖抑制率、凋亡率、Cleaved-caspase-3蛋白水平、细胞培养上清液中LDH和MDA水平（P<0.05）,升高miR-490-3p水平、SOD活性（P<0.05）。结论 沉默lncRNA FIRRE可能通过靶向上调miR-490-3p抑制H₂O₂诱导的H9c2细胞凋亡和氧化应激。更多还原

【Abstract】 Objective To investigate the effect of lncRNA FIRRE on the apoptosis and oxidative stress of cardiomyocytes H9c2 induced by hydrogen peroxide（H₂O₂）.Methods H9c2 cells were transfected with si-lncRNA FIRRE,miR-490-3p mimics or co-transfected with si-lncRNA FIRRE and anti-miR-490-3p, and then treated with 200 μmol/L H₂O₂ for 24 h.The relative expression of lncRNA FIRRE and miR-490-3p were detected by RT-qPCR,the inhibitory rate of cell proliferation was detected by CCK-8,the apoptosis was detected by flow cytometry, and MDA and LDH levels and SOD activity were detected by the kit.Meanwhile, dual-luciferase reporter assay was used to verify the targeting relationship between lncRNA FIRRE and miR-490-3p.Cleaved-caspase-3 protein expression levels were determined by Western blotting.Results After H9c2 cells was treated with 200 μmol/L H₂O₂ for 24 h, the expression of lncRNA FIRRE was increased（P<0.05）,as well as the cell proliferation inhibition rate, the apoptosis rate, Cleaved-caspase-3 protein level, LDH and MDA level（P<0.05）,while the expression level of miR-490-3p and SOD activity were decreased（P<0.05）.Silencing lncRNA FIRRE or overexpression of miR-490-3p could reduce cell proliferation inhibition rate, apoptosis rate, Cleaved-caspase-3 protein level, LDH level in cell culture supernatant and MDA content in cells（P<0.05）,increased the level of miR-490-3p and SOD activity（P<0.05）.Conclusion Silencing lncRNA FIRRE may inhibit H₂O₂-induced H9c2 cell apoptosis and oxidative stress by up-regulating miR-490-3p.更多还原