【摘要】 前列腺特异性抗原(PSA)是前列腺癌的血清生物标志物，对其含量的测定在前列腺癌的早期诊断和治疗中十分重要。表面增强拉曼光谱(SERS)技术，拥有极高的灵敏度和极强的选择性，在抗体抗原检测研究中逐渐受到重视。研究提出了一种基于SERS的生物传感器方法对PSA进行定量检测。使用溅射手段制备了均匀的SERS活性基底，使用PSA适配体代替抗体进行PSA识别，通过探针分子亚甲基蓝(MB)信号的降低来进行PSA检测，为PSA检测和诊断提供了方法。更多还原

【Abstract】 Prostate specific antigen(PSA) is a serum biomarker of prostate cancer. The determination of its content is very important for the early diagnosis and treatment of prostate cancer. Surface enhanced Raman spectroscopy(SERS) has attracted much attention in antigen detection due to its high sensitivity and selectivity. In this study, we developed a SERS-based biosensor for quantitative detection of PSA. A uniform SERS active substrate was prepared by sputtering and the aptamer of PSA was used instead of antibody for PSA recognition. PSA detection was performed by reducing the signal of probe molecule methylene blue, which provided a method for PSA detection and diagnosis.更多还原