【摘要】 胰岛素自身抗体（IAA）是1型糖尿病（T1DM）的重要生物标志物。IAA早在患者出现临床表现之前就存在于血清中，因此对早期筛查T1DM具有重要的意义。然而，目前现有的检测方法耗时长，缺乏敏感性和特异性。在此背景下，设计了一种基于IAA的表面增强拉曼散射（SERS）免疫芯片。随着IAA浓度的增加，对巯基苯甲酸（MBA）在～1 074 cm^-1处的特征峰会逐渐发生谱峰位移，因此利用特征峰拉曼位移的变化可实现对IAA的定量分析。实验结果表明该方法在临床应用中具有快速、敏感和准确早期筛查T1DM的潜力。更多还原

【Abstract】 Insulin autoantibody（IAA） is an important biomarker of type 1 diabetes mellitus（T1DM）. IAA is present in the serum long before the clinical manifestations of patients, so they have important significance for early screening of T1DM. However, current detection methods are time-consuming and lack sensitivity and specificity. In this context, we developed an IAA-based surface-enhanced Raman scattering（SERS） immunochip. With the increase of IAA concentration, the characteristic peak of p-mercaptobenzoic acid（MBA） at ～1 074 cm^-1 gradually shifted, so the change of Raman shift of characteristic peak can be used for the quantitative analysis of IAA. The experimental results show that this method has the potential for rapid, sensitive, and accurate early screening of T1DM in clinical application.更多还原