【摘要】 线粒体内细胞色素c存在可相互转化的还原和氧化态两种分子形式。基于之前的研究，发现镍纳米粒子可以作为电子供体特异性还原细胞色素c。镍纳米材料具有一定的表面增强拉曼散射增强能力，但其增强因子较小，限制了其应用。在该研究中，成功合成了Ag@Ni核壳纳米粒子，借助于Ag核的拉曼信号增强能力和Ni壳的电子供体特性，该SERS基底可用于氧化还原蛋白的电子传递研究。更多还原

【Abstract】 Cytochrome c exists in two interconvertible reduced and oxidized forms in mitochondria. Based on previous studies, nickel nanoparticles were found to act as electron donors to specifically reduce cytochrome c. Nickel nanomaterials have a certain ability to enhance Raman scattering, but their enhancement factor isrelatively small, which limits their applications. In this study, Ag@Ni core-shell nanoparticles were successfully synthesized. With the help of the Raman signal enhancement ability of the Ag core and the electron-donor properties of the Ni shell, the SERS substrate can be used for electron transport studies of redox proteins.更多还原