【摘要】 表面增强拉曼散射(SERS)技术是一种在单分子水平上通过收集分子光谱信号来识别分子物种的强有力的工具，现已被广泛用于医学诊断、食品安全、生物分析等领域。本文综述了SERS技术在常见细菌及病原体的研究进展，阐述了通过设计和调控纳米结构的形貌、种类(贵金属和半导体衬底)、掺杂元素、氢化时间和物理条件(温度、pH)等使其具有优异的灵敏度，从而扩大SERS技术的应用。同时讨论了SERS技术与现在热门的微流控技术、CRISRP技术的联用，以及在单分子检测上的优缺点。最后，展望了SERS生物传感器技术的发展前景。更多还原

【Abstract】 Surface-enhanced Raman scattering(SERS) technology is a powerful tool to identify molecular species by collecting molecular spectral signals at the single-molecule level, and has been widely used in medical diagnosis, food safety, biological analysis and other fields. In this paper, the research progress of SERS technology in common bacteria and pathogens was reviewed, and it was expounded that the morphology, type(precious metal and semiconductor substrate), doping elements, hydrogenation time, physical conditions(temperature, pH) of nanostructures were designed and regulated to make it have excellent sensitivity, thus amplifying the application of SERS technology. At the same time, the combination of SERS technology and popular microfluidic technology and CRISRP technology were discussed, as well as the advantages and disadvantages of SERS technology in single molecule detection. Finally, the development prospect of SERS biosensor technology was prospected.更多还原