【摘要】 蚯蚓天然免疫系统对病原菌具有良好的抑制效果，但在蚯蚓生物滤池（VF）工艺中蚯蚓的免疫功能需要进一步探究。为此，以无蚯蚓的生物滤池（BF）为平行对照，考察蚯蚓在生物滤池胁迫条件下的抑菌能力；同时，以牛粪饲养的蚯蚓（CDW）为对照，以活性物质（体腔液和粗脂质）为表征指标，分析VF中蚯蚓的抑菌能力变化，并通过GC/MS和iTRAQ技术分析了两种蚯蚓粗脂质中脂肪酸的组成差异，以及体腔液中发挥抑菌功能的蛋白组分并进行鉴定注释。结果表明，采用VF处理剩余污泥时对11种病原菌及指示菌的平均去除率为58.0%，较BF高出17.8%,VF中蚯蚓的抑菌能力增强；与CDW相比，VF中蚯蚓脂肪酸的抑菌能力无明显差异，但其体腔液对病原菌的抑制能力提高了14.1%；与CDW相比，VF中蚯蚓受到了连续的污泥冲击、病原菌丰度大等胁迫，但其具有更高的代谢活性和更强的免疫反馈，分子质量在3～10、10～30 ku范围的抑菌蛋白表达显著提高。更多还原

【Abstract】 The natural immune system of earthworm has a good inhibitory effect on pathogens.However, the immune function of earthworm in vermifiltration(VF) process needs further investigation. In this paper, the antibacterial ability of earthworms under stress conditions in biofilter(BF) was investigated by using a BF without earthworms as parallel control group. In addition, the changes in antibacterial ability of earthworms in VF were analyzed by using earthworms cultivated by cow dung earthworm(CDW)as control and active substances(coelomic fluid and crude lipid) as indicators, and the difference of fatty acid composition in crude lipids of two kinds of earthworms and their antibacterial protein components in coelomic fluid were analyzed and identified by GC/MS and iTRAQ. The average removal rates of 11 kinds of pathogens and indicator bacteria in VF for treatment of excess sludge was 58.0%, which was 17.8%higher than that in BF, indicating that the antibacterial ability of earthworms in VF was enhanced.Compared with CDW, there was no significant difference in antibacterial ability of earthworm fatty acids in VF, but the antibacterial ability of coelomic fluid to pathogens increased by 14.1%. Compared with CDW, earthworms in VF were subjected to stress such as continuous sludge shock and high abundance of pathogens. However, they had higher metabolic activity and stronger immune feedback, and the expression of antibacterial proteins with molecular weights in the ranges of 3-10 ku and 10-30 ku was significantly increased.更多还原