【摘要】 目的：研究槲皮素对糖尿病大鼠肾脏的保护作用。方法：将SD大鼠随机分为四组：正常对照组、槲皮素对照组、糖尿病组、槲皮素治疗组，收集大鼠血液与尿液标本，检测肾脏损伤相关指标，Western-blot法检测大鼠肾皮质纤连蛋白（Fibronectin,FN）的表达。结果：糖尿病组血糖≥16.7 mmol·L-1提示糖尿病肾病模型建立成功。与正常对照组比较，糖尿病组肾重/体重、血糖、尿糖、血肌酐、血尿素氮、尿量、尿总蛋白增加，尿肌酐降低，差异有统计学意义（P<0.05）。与糖尿病组比较，槲皮素治疗组肾重/体重、血糖、血肌酐、血尿素氮、尿量、尿总蛋白降低，尿肌酐增加，差异有统计学意义（P<0.05）。与正常对照组相比，糖尿病组肾小球基底膜出现弥漫性加厚及肾脏胶原的堆积明显增多，槲皮素治疗后肾小球基底膜弥漫性增厚及胶原蛋白堆积出现明显改善。与正常对照组比较，糖尿病组FN蛋白表达量显著增加（P<0.01）；与糖尿病组比较，槲皮素治疗组组FN表达量显著减少（P<0.01）。结论：槲皮素可以延缓链脲佐菌素（Streptozotocin,STZ）诱导的糖尿病大鼠肾脏的损伤，并且能够抑制胞外基质蛋白FN的表达，对STZ所致糖尿病大鼠肾脏具有一定的保护作用。更多还原

【Abstract】 Objective : To investigate the protective effect of quercetin on diabetic nephropathy rats. Methods : The SD rats were randomly divided into four groups: normal control group, quercetin control group, diabetic nephropathy group, quercetin treatment group. The renal injury-related indicators in serum and urine were detected. The expression of fibronectin in the renal cortex was investigated by Western blot. Results : Blood glucosein diabetic group≥16. 7 mmol·L-1 indicated that the model was successfully established. Compared with normal control group, the kidney weight/bodu weight, the blood glucose, glucose, blood creatinine, blood ureanitrogen, urine volume, urine protein in diabetic group increased, and urine creatinine decreased, with statistical significance(P<0. 05). Compared with diabetic nephropathy group, the kidney weight/bodu weight, the blood glucose, blood creatinine, blood urea nitrogen, urine volume, urine protein in quercetin treatment group decreased, and urine creatinine increased, with statistical significance(P<0. 05). Compared with normal control group, diffuse dthickening of glomerular basement membrane was seen in diabetic group, and the accumulation of collagenin the kidneys was significantly increased. After quercetin treatment,diffused thickening of glomerular basement membraneand the accumulation of collagen in the kidneys were significantly improved. Compared with normal control group,the expressionof FN protein in diabetic nephropathy group was significantly increased(P<0. 01). Compared with diabetic nephropathy group, theexpression of FN in quercetin treatment group was significantly decreased(P<0. 01). Conclusion : Quercetin can ameliorate renal injury and significantly inhibit the fibronectin expression of diabetic nephropathy rats to protect the kidney of diabetic rats from injury.更多还原