【摘要】 目的：探索子宫内膜癌(EC)中长链非编码RNA KRT7-AS的表达及其对HEC-1A细胞增殖的影响。方法：采用在线数据库检测分析KRT7-AS在EC及癌旁正常组织中的表达；用RT-qPCR法检测人子宫内膜癌细胞系HEC-1A、HEC-1B、KLE、B-MD-1C细胞中KRT7-AS的表达；分别构建KRT7-AS敲低(shKRT7-AS)和对照(shCtrl)慢病毒。慢病毒感染HEC-1A细胞并培养5 d后，采用RT-qPCR检测敲低效率，MTT法检测细胞增殖，流式细胞术测定细胞周期和凋亡。结果：在EC组织中，KRT7-AS表达高于癌旁组织(P<0.05),KRT7-AS高表达患者的总生存数低于低表达患者(P<0.05)。KRT7-AS敲低组的HEC-1A在第5天增殖速率显著降低(P<0.05)。KRT7-AS敲低导致HEC-1A细胞停滞于G0/G1期(P<0.05);细胞凋亡比例则显著增加(P<0.05)。结论：KRT7-AS异常高表达促进EC的发展。更多还原

【Abstract】 Objective: To explore the expression of long non-coding RNA KRT7-AS in endometrial carcinoma(EC) and its effect on the proliferation of HEC-1A cells. Methods: The online database was used to analyze the expression of KRT7-AS in EC and normal tissue adjacent to cancer; RT-qPCR was used to detect the human endometrial cancer cell lines KLE, HEC-1A, HEC-1B, B-MD-1C expression in KRT7-AS cells; KRT7-AS knockdown(shKRT7-AS) and control(shCtrl) lentiviruses were constructed, respectively. After lentivirus-infected HEC-1A cells and cultured for 5 days, the knockdown efficiency was detected by RT-qPCR, cell proliferation was detected by MTT method, and cell cycle and apoptosis were detected by flow cytometry. Results: The expression of KRT7-AS in EC tissue was significantly higher than that in adjacent tissue(P<0.05), and the overall survival rate of patients with high KRT7-AS expression was significantly lower than that of patients with low KRT7-AS expression(P<0.05). The proliferation rate of HEC-1A in the KRT7-AS knockdown group was significantly decreased on the 5th day(P<0.05). KRT7-AS knockdown caused HEC-1A cells to arrest in G0/G1 phase(P<0.05), while the proportion of apoptosis was significantly increased(P<0.05). Conclusion: The abnormally high expression of KRT7-AS may promote the development of EC.更多还原