【摘要】 为了验证血浆外泌体作为检测结直肠癌的新型生物标记物的可行性，设计了一种太赫兹超材料生物传感器。采用基于有限积分时域方法的全波电磁仿真软件进行器件结构设计，并采用表面微加工工艺制造样片。为了提高二氧化硅表面的粘接性能，采用硅烷偶联剂APTES来增强粘附性，使用金纳米粒子(AuNPs)修饰生物传感器表面。通过配体交换法，在AuNPs表面引入羧基，将异双官能团聚乙二醇(HS-PEG-COOH)组装到AuNPs上。随后，修饰对外泌体具有特异性的热休克蛋白60(HSP60)抗体。按照外泌体分离和纯化试剂盒的说明提取实验所需外泌体，并使用透射电子显微镜、蛋白质印迹法和纳米颗粒跟踪分析法进行鉴定。通过对结直肠癌患者血浆衍生的外泌体和健康人群血浆衍生的外泌体进行比较，发现传感器的共振峰频移明显。更多还原

【Abstract】 In order to verify the feasibility of plasma exosomes as a novel biomarker for the detection of colorectal cancer, a terahertz metamaterial biosensor was designed. The terahertz metamaterial biosensor was designed using a full wave electromagnetic simulation software based on the finite integration time domain method and fabricated by a surface micromachining process. First, the application of the silane coupling agent APTES on the surface of silica improves the adhesion performance of the surface of silica, and Au-Nanoparticles(AuNPs) were used to modify the biosensor surface. Then, by the ligand exchange method, carboxyl groups were introduced on the surface of AuNPs, and the heterobifunctional polyethylene glycol(HS-PEG-COOH) was assembled on the AuNPs. Subsequently, the heat shock protein 60(HSP60) antibody specific for exosomes was modified. Exosomes were extracted by the instructions of Exosomes Isolation and Purification Kit, and identified by using transmission electron microscope, Western blot, and nanoparticle tracking analysis. The biosensor covered with plasma derived exosomes of colorectal cancer patients showed different resonant frequency shift from that with healthy controls derived exosomes.更多还原