【摘要】 目的 动态监测河南省许昌市本土流行的新冠病毒毒株组成，及时发现新变异株，评估变异株的传播力、致病力和免疫逃逸能力等生物学特性，为疫情防控提供技术支撑。方法 采用高通量测序方法对2023年2月15-19日来自哨点医院的6例新冠病毒感染者的咽拭子标本进行全基因测序，进行序列对比和进化分析。结果 6例新冠病毒感染者样本进行全基因测序，获得6条长度为29 844～29 853 bp序列。经过Nextclade V2.11.0在线分析，6条序列均属于新冠病毒奥密克戎BA.5变异株，其中病例1为BF.7.14，其余均为BA.5.2.48分支。进化树显示6条序列位于奥密克戎BA.5进化分支，与河南省其他地市BA.5进化株位于同一分支。未发现BQ、XBB等变异株。结论 测序的6例本土病例新冠病毒均为奥密克戎变异株，BA.5.2.48分支变异株依然是许昌市优势流行毒株。更多还原

【Abstract】 Objective To dynamically monitor the composition and distribution of local circulating SARS-CoV-2strains in Xuchang city,Henan province,timely discover new variants,and evaluate the biological characteristics changes and risks of the variants,such as transmissibility,pathogenicity and immune escape ability,by which providing technical support for epidemic prevention and control. Methods Whole gene sequencing was performed on throat swabs of 6 patients infected with SARS-CoV-2 from sentinel hospitals during February 15 to 19,2023,and the sequences were compared and analyzed by high-throughput sequencing method. Results The six samples from SARS-CoV-2 infected patients were sequenced,with the length of 29 844-29 853 bp. The sequences were analyzed online by Nextclade V2.11.0,all of them belonged to SARS-CoV-2 Omicron BA.5 variants,among which,case 1 was BF.7.14 and the others were BA.5.2.48. The phylogenetic tree analysis showed that the 6 sequences were located in the clade of Omicron BA.5,which was in the same clade as Omicron BA.5 strains in other cities of Henan Province. No mutant strains such as BQ and XBB were found. Conclusion The genome of 6 local cases of SARS-CoV-2 was all Omicron variant strains,and the BA.5.2.48 branch variant was still the advantage epidemic strain in Xuchang city.更多还原