【摘要】 为揭示盐碱环境下盐爪爪内生菌群落结构组成及其多样性，以新疆5个典型盐碱地区的盐爪爪为研究对象，采用高通量测序技术分别对其根部和地上部分样品的内生菌进行分析。结果表明：获得的366个细菌操作分类单元(Operational taxonomic units, OTUs)共涉及16个门，70个目，321个属；以变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)为主要菌门。获得的869个真菌OTUs共涉及14个门，86个目，291个属；以子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、油壶菌门(Olpidiomycota)为主要菌门。其中，嗜盐单胞菌属(Halomonas)和链格孢属(Alternaria)占绝对优势。不同地区和不同组织的内生细菌群落和内生真菌群落组成及群落丰富度差异显著。土壤中的钠离子(Na~+)、氯离子(Cl~-)和电导率(EC)与内生菌主要门的分布呈显著正相关关系，表明不同地区内生群落的构成差异主要与土壤盐碱化相关。研究初步揭示了新疆盐碱地区盐爪爪内生菌群落结构组成与分布格局，对阐明内生菌在生态系统的演替、生物多样性及其生态功能具有科学意义。更多还原

【Abstract】 To investigate the composition and distribution of endophytic community of Kalidium foliatum in the salt-alkali environment, samples were collected from five regions in Xinjiang, and the community composition and diversity of endophytes was analyzed by the high-throughput sequencing method. The results showed that the 366 bacterial operational taxonomic units(OTUs) belonged to 16 phyla, 70 orders and 321 genera. Proteobacteria, Firmicutes and Actinobacteria are the dominant phyla. A total of 869 fungal OTUs were obtained for 14 phyla, 86 orders and 291 genera. Ascomycota, Basidiomycota and Olpidiomycota were the major phyla. Among them, Halomonas and Alternaria were dominant genera. The endophytic community composition and community richness varied significantly among different areas and tissues. The distribution of sodium ions(Na~+), chloride ions(Cl~-) and electrical conductivity(EC) in soil was positively correlated with the distribution of the main phyla. The composition differences of endophytic communities in different regions were related to soil salinization. This study preliminarily revealed the community structure, composition and distribution pattern of endophytes in Kalidium foliatum in the saline-alkali regions of Xinjiang, which was of great scientific significance to further elucidate the succession, biodiversity and ecological functions of endophytes in ecosystems.更多还原