【摘要】 目的 研究泥鳅（Misgurnus anguillicaudatus）外周血细胞的细胞化学染色特征及细胞化学谱。方法 自泥鳅尾静脉取血，立即制作血涂片，进行瑞氏及7种细胞化学染色，包括酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、氯乙酸AS-D萘酚酯酶（AS-D）、α-醋酸萘酚酯酶（α-NAE）、过碘酸-雪夫（PAS）、过氧化物酶（POX）和苏丹黑B(SBB)染色。结果 在泥鳅外周血中分辨出红细胞、4种白细胞和血栓细胞，4种白细胞分别为淋巴细胞、单核细胞、嗜中性粒细胞和嗜酸性粒细胞。红细胞PAS染色呈阳性，ACP、AKP、AS-D、α-NAE、POX和SBB染色均为阴性；淋巴细胞AS-D、α-NAE和PAS染色呈阳性，ACP、AKP、POX和SBB染色为阴性；单核细胞AS-D染色呈阳性，ACP、α-NAE和PAS染色为弱阳性，AKP、POX和SBB染色为阴性；嗜中性粒细胞ACP和AS-D呈阳性染色，α-NAE、PAS和SBB染色为弱阳性，AKP和POX染色为阴性；嗜酸性粒细胞ACP和AS-D染色呈强阳性，α-NAE、PAS和SBB染色为阳性，AKP和POX染色为阴性；血栓细胞AS-D染色呈阳性，α-NAE和PAS染色为弱阳性，ACP、AKP、POX和SBB染色为阴性。结论 泥鳅外周血细胞的细胞化学谱和其他鱼类有相同之处，也有一些明显的物种特异性。本研究结果为泥鳅的健康检测和疾病诊断提供了基础资料。更多还原

【Abstract】 Objective To study the cytochemical staining characteristics and cytochemical profiles of peripheral blood cells in the loach(Misgurnus anguillicaudatus). Methods Blood was drawn from the caudal vein of the loach and immediately used to prepare blood smears, which were then subjected to Wright’s staining and seven types of cytochemical stains, including acid phosphatase(ACP), alkaline phosphatase(AKP), chloroacetate AS-D naphthol Esterase(AS-D), α-naphthyl acetate esterase(α-NAE), periodic acid-Schiff(PAS), peroxidase(POX), and Sudan black B(SBB) staining. Results Red blood cells, four types of white blood cells, and thrombocytes were identified in the peripheral blood of the loach. The four types of white blood cells were lymphocytes, monocytes,neutrophils, and eosinophils. Red blood cells showed positive PAS staining but were negative for ACP, AKP, AS-D, α-NAE, POX, and SBB staining; lymphocytes were positive for AS-D, α-NAE, and PAS staining, but negative for ACP, AKP, POX, and SBB staining;monocytes were positive for AS-D staining, weakly positive for ACP, α-NAE, and PAS staining, and negative for AKP, POX, and SBB staining; neutrophils were positive for ACP and AS-D staining, weakly positive for α-NAE, PAS, and SBB staining, and negative for AKP and POX staining; eosinophils were strongly positive for ACP and AS-D staining, positive for α-NAE, PAS, and SBB staining,and negative for AKP and POX staining; thrombocytes were positive for AS-D staining, weakly positive for α-NAE and PAS staining,and negative for ACP, AKP, POX, and SBB staining. Conclusion The cytochemical profiles of peripheral blood cells in the loach share similarities with other fish species, but also exhibit some clear species-specific characteristics. The results of this study provide fundamental data for health monitoring and disease diagnosis in loaches.更多还原