【摘要】 目的 分析转录因子婆罗双树样基因4(spalt-like transcription factor 4, SALL4)表达水平改变对膀胱尿路上皮癌细胞增殖、迁移及侵袭能力的影响，明确SALL4与膀胱尿路上皮癌上皮间质转化（epithelial mesenchymal transition, EMT）的关系。方法 CCK-8法及Edu染色检测细胞增殖，Transwell分析检测细胞迁移和侵袭，Western blot检测SALL4及EMT相关蛋白和β-catenin水平。结果 过表达SALL4促进膀胱尿路上皮癌细胞增殖、迁移及侵袭，增加N-cadherin、vimentin、Snail和β-catenin水平，减少E-cadherin水平，敲低SALL4表达具有相反作用。结论 SALL4可能通过激活Wnt/β-catenin信号通路促进膀胱癌细胞EMT过程，进而促进膀胱尿路上皮癌细胞增殖、迁移及侵袭。更多还原

【Abstract】 Objective To clarify the relationship between spalt-like transcription factor 4(SALL4) and epithelial mesenchymal transition(EMT) of bladder urothelium cancer cells by analyzing the effect of changing expression level of SALL4 on the proliferation,migration and invasion of bladder urothelium cancer cells. Methods CCK-8 and Edu staining were used to detect cell proliferation,Transwell analysis was used to detect cell migration and invasion, Western blot was used to detect the levels of SALL4, EMT related proteins and β-catenin. Results The overexpression of SALL4 promoted the proliferation, migration and invasion of bladder urothelium cancer cells, and increased the levels of N-cadherin, vimentin, Snail and β-catenin, and reduced E-cadherin levels, while knocking down SALL4 expression had the opposite effect. Conclusion SALL4 may promote the EMT process of bladder cancer cells through activating Wnt/β-catenin signaling pathway, thereby promoting the proliferation, migration and invasion of bladder urothelial cancer.更多还原