【摘要】 材料表面浸润性和表面荷电对微生物在材料表面的附着有着非常重要的影响.本文采用静电纺丝法在不锈钢电极表面分别制备了疏水和亲水的聚苯胺纤维涂层,利用电化学阻抗谱监测大肠杆菌在两种聚苯胺纤维涂层表面的附着情况.结果表明,亲水表面有利于抑制大肠杆菌的附着.通过电化学极化使亲水表面分别带正电荷和负电荷,利用平板涂布计数法研究了不同极化时间下两种荷电表面的抑菌率,发现无论正电荷还是负电荷都有利于抑制微生物附着,而且二者没有明显差异.在相同的阴极极化条件下,亲水和疏水的聚苯胺纤维涂层抑菌性能相差不大,说明对于电纺聚苯胺涂层抑制细菌附着而言,电化学极化的影响大于表面浸润性的影响.更多还原

【Abstract】 The surface wettability and surface charge of the materials have a very important influence on the attachment of microorganisms to the material surfaces. In this paper, two kinds of polyaniline fiber coatings, hydrophobic and hydrophilic, were prepared on the surface of stainless steel electrodes by electrospinning. Electrochemical impedance spectroscopy was used to monitor the adhesion of Escherichia coli(E. coli) on the surfaces of the two polyaniline fiber coatings, and the results showed that the hydrophilic surface was beneficial to inhibit the adhesion of E. coli.Further, the hydrophilic surface was positively and negatively charged, respectively, by electrochemical polarization, and the bacteriostatic rate of two kinds of charge surface under different polarization times were studied by plate count method, and it was found that both positive and negative charges were beneficial to inhibit the attachment of microorganisms, and there was no significant difference between them. Moreover, under the same cathodic polarization conditions, the antibacterial properties of hydrophilic and hydrophobic polyaniline fiber coatings were not much different, indicating that the effect of electrochemical polarization on the inhibition of bacterial adhesion by electrospun polyaniline coatings is greater than that of surface wettability.更多还原