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基于BSA-seq的水稻籽粒耐陈化QTL定位分析

QTLs Mapping Analysis of Rice Grain Aging Tolerance Based on BSA-seq

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【作者】 王雪彬张健韦燕燕罗继景梁云涛蔡中全

【Author】 Wang Xuebin;Zhang Jian;Wei Yanyan;Luo Jijing;Liang Yuntao;Cai Zhongquan;College of Agricultural, Guangxi University;College of Life Science and Technology, Guangxi University;Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences;Agricultural and Animal Husbandry Development Research Institute, Guangxi University;

【通讯作者】 梁云涛;蔡中全;

【机构】 广西大学农学院广西大学生命科学与技术学院广西农科院水稻研究所,广西水稻遗传育种重点实验室广西大学农牧产业发展研究院

【摘要】 籽粒耐陈化是水稻耐储藏特性之一,也是重要的优势农艺性状。定位籽粒耐陈化QTL位点,对该性状的应用有重要作用。以野生稻与栽培稻杂交后代耐储材料‘S18Z246’和不耐储的‘日本晴’(Nip)杂交,构建耐储存特性遗传研究F2世代群体;使用高温高湿环境(温度为50℃,湿度为80%)陈化后发芽率变异量化耐陈化能力;构建两个极端表型混池,采用BSA-seq方法,发现了qRSS-1-1、qRSS-1-2、qRSS-1-3、qRSS-2-1、qRSS-2-2等5个QTL及其In Del标记。其中qRSS-1-3是新发现的QTL。研究结果为水稻籽粒耐储存性状的进一步研究和应用奠定了基础。

【Abstract】 Rice grain aging tolerance is a reflection of its storage tolerance and an important agronomic trait.Mapping QTLs locus for aging tolerance is important for the application of this trait. The F2generation population for genetic research on storage tolerance was constructed by crossing the storage-tolerant material ’S18Z246’ of the offspring of wild rice and cultivated rice with the storage-intolerant material ’Nip’. Using high temperature and humidity(50 ℃, 80% humidity) environment to quantify the aging tolerance of germination rate variation. Two extreme phenotypic mixed pools were constructed and BSA-SEQ method was used to identify five QTLS and their InDel markers, namely qRSS-1-1, qRSS-1-2, qRSS-1-3, qRSS-2-1 and qRSS-2-2. Among them, qRSS-1-3 is the newly discovered QTL. These results lay a foundation for further research and application of rice grain storage tolerance.

【关键词】 水稻耐储存陈化QTL定位
【Key words】 RiceStorage ToleranceAgingQTL mapping
【基金】 广西水稻遗传育种重点实验室开放课题(160-380-16-7; 2018-15-Z06-KF14);广西农业科学院基本科研业务专项(桂农科2020YM04);广西农业科学院科技发展基金项目(桂农科2020YM121)共同资助
  • 【文献出处】 分子植物育种 ,Molecular Plant Breeding , 编辑部邮箱 ,2023年16期
  • 【分类号】S511
  • 【下载频次】842
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