【摘要】 研究以百子莲(Agapanthus praecox ssp. orientalis)茎尖组织为材料，利用RACE (cDNA末端快速扩增)方法，获得百子莲VIN3基因c DNA全长序列，命名为ApVIN3,GenBank登录号为KJ472789。序列分析表明，百子莲ApVIN3基因cDNA全长1 943 bp，其中开放阅读框为1 680 bp，编码559个氨基酸，5’非编码区(5’UTR)和3’非编码区(3’UTR)分别为88和175 bp。ApVIN3编码一个具有C4HC3 (cys4-His-cys3)保守序列结构的PHD-finger蛋白，与二穗短柄草(XP＿003573553.1) VIN3蛋白有很高的相似性。实时荧光定量分析表明，ApVIN3在百子莲茎尖中的表达量受温度调控的影响，其表达量随着低温时间的延长逐渐上升。ApVIN3可能在百子莲成花诱导过程中发挥着重要的作用。更多还原

【Abstract】 A core fragment highly homologous with VERNALIZATION INSENSITIVE 3(VIN3) was obtained on the basis of preliminary Agapanthus praecox ssp. orientalis transcriptome sequencing results. The full-length cDNA sequence of VIN3 gene in A. praecox ssp. orientalis was obtained by RACE method, which was named ApVIN3with the accession number of KJ472789. The results of sequence analysis showed that the full length of ApVIN3cDNA was 1 943 bp, which contained an open reading frame of 1 680 bp, 559 encoding amino acids, untranslated region(UTR) 5’ and 3’ with the length of 88 and 175 bp, respectively. ApVIN3 encoding a PHD-finger protein with a conserved sequence structure of C4HC3(cys4-His-cys3), which was highly similar to the VIN3 protein of Brachypodium distachyum(L.) Beauv(XP＿003573553.1). Quantitative Real-time PCR showed that the expression of ApVIN3 gene in stem tip of A. praecox ssp. orientalis was affected by temperature regulation, and the expression lev el increased gradually with the extension of low temperature time. This gene may play an important role in the floral induction process of A. praecox ssp. orientalis, and its function and regulation mechanism can be studied deeply.更多还原