【摘要】 茉莉酸甲酯能诱导广藿香叶片中百秋李醇含量升高，但是其中的分子机制不清楚。本研究从茉莉酸甲酯处理的广藿香叶片转录组数据中筛选到一个表达量显著增加的基因，经过序列比对发现该基因与唇形科丹参转录因子Sm TIFY6b具有高度同源性，因此将其命名为PatTIFY6b。通过生物信息学对PatTIFY6b编码蛋白的结构和功能进行分析和预测，并以广藿香cDNA为模板，克隆得到PatTIFY6b。PatTIFY6b定位于细胞核，在广藿香不同组织中均有表达，叶片中表达量最高。茉莉酸甲酯处理下，PatTIFY6b和百秋李醇合成途径基因具有相似的表达模式，说明茉莉酸甲酯可能通过PatTIFY6b调控百秋李醇的合成。利用原核表达系统，成功表达了PatTIFY6b蛋白。将PatTIFY6b过表达在双子叶模式植物拟南芥中，筛选到纯合株系PatTIFY6b-ox-17。本研究首次克隆广藿香PatTIFY6b基因并对其功能进行初步探究，为后期研究广藿香百秋李醇生物合成的分子调控机制提供理论基础。更多还原

【Abstract】 Methyl jasmonate can promote the accumulation of patchouli alcohol, but molecular mechanism is unclear. In this study, we screened an up-regulated gene from transcriptome of Pogostemon cablin treated with methyl jasmonate, named PatTIFY6b due to its sequence similarity to SmTIFY6b of Salvia miltiorrhiza. The protein encoded by PatTIFY6b were analyzed and predicted by bioinformatics, and full length of PatTIFY6b was cloned. PatTIFY6b localized in the nucleus and expressed in different tissues of Pogostemon cablin. In leaves, PatTIFY6b has the highest level of expression compared with that in other tissues. Upon methyl jasmonate treatment,PatTIFY6b and the genes related with patchouli alcohol synthesis have similar patterns of expression, suggesting that methyl jasmonate could regulate the synthesis of patchouli alcohol through PatTIFY6b. PatTIFY6b encodes18.6 kD protein and can be successfully induced in prokaryotic system. Overexpressed PatTIFY6b in Arabidopsis thaliana, and screened the homozygous line PatTIFY6b-ox-17. In this study, we cloned the PatTIFY6b and studied its function firstly, which provided a theoretical basis for the molecular mechanism of patchouli alcohol biosynthesis.更多还原