【摘要】 建立了超声辅助分散液液微萃取（US-DLLME）-高效液相色谱（HPLC）法测定环境水样中马兜铃酸Ⅰ（AA-Ⅰ）和马兜铃酸Ⅱ（AA-Ⅱ）的方法。考察了萃取溶剂和分散剂的种类及用量、超声时间、料液相pH值和盐效应对AA-Ⅰ和AA-Ⅱ富集因子的影响。在最佳萃取条件下，AA-Ⅰ和AA-Ⅱ的富集因子高达95.9和93.4。本方法检测AA-Ⅰ和AA-Ⅱ的线性范围均为0.01～2.00μg/mL，相关系数（R2）分别为0.9994和0.9997，检出限（LOD）分别为2.5和3.0 ng/mL，定量限（LOQ）分别为8.0和9.5 ng/mL。本方法简单、快速，前处理过程仅需12 min。采用本方法对浏阳河水样和湘江水样进行检测，加标回收率在91.7%～97.8%之间，相对标准偏差（RSD）均小于5.7%。本方法环境友好、灵敏度高，适用于环境水样中AA-Ⅰ和AA-Ⅱ的快速测定。更多还原

【Abstract】 A novel method was developed for analysis of aristolochic acid Ⅰ and Ⅱ(AA-Ⅰ and AA-Ⅱ) in environmental water by ultrasonic-assisted dispersive liquid-liquid microextraction(US-DLLME) coupled with high performance liquid chromatography(HPLC). The effects of kinds of the extraction solvent and the disperser solvent, the volumes of the extraction solvent and the disperser solvent, the ultrasonic time, the pH value and salt addition in the feed solution on enrichment factor were investigated. Under the optimal extraction conditions, the enrichment factors of up to 95.9 for AA-Ⅰ and 93.4 for AA-Ⅱ were obtained. The established method showed good linearity for both AA-Ⅰ and AA-Ⅱ in the concentration range from 0.01 to 2.00μg/mL with correlation coefficients(R2) of 0.9994 and 0.9997, respectively. The limits of detection(LODs) were 2.5 and 3.0 ng/mL, and the limits of quantitation(LOQs) were 8.0 and 9.5 ng/mL for AA-Ⅰ and AA-Ⅱ, respectively. The pretreatment procedure was completed within 12 min. The recoveries of AA-Ⅰ and AA-Ⅱ in spiked samples were from 91.7%to 96.9% with relative standard deviations(RSDs) less than 5.7% for Liuyang River samples. The recoveries of AA-Ⅰ and AA-Ⅱ in spiked samples were from 91.9% to 97.8% with RSDs less than 5.1% for Xiangjiang River samples. The results demonstrated that the proposed method was sensitive, fast and environmental-friendly, and could be suitable for analysis of AA-Ⅰ and AA-Ⅱ in environmental water.更多还原