【摘要】 目的 应用傅里叶变化红外光谱（Fourier Transform Infrared,FTIR）技术和化学计量学方法鉴定尸体内外精液（斑）的留存时间。方法 利用猪阴道模拟尸体内环境，取新鲜精液分别留存于体内外0～168 h，采集红外光光谱数据，通过化学计量学方法建立主成分分析（Principal Components Analysis, PCA）和偏最小二乘（Partial Least Squares, PLS）回归模型。结果 体内外精液（斑）红外光谱峰位随时间变化不明显，PCA结果有较好的聚类关系，在PLS回归模型中预测的偏差值为15.786 3 h,R²为0.922 4,CARS算法选取的特征波数点位于1 000～1 180、1430～1 480、1 700～1 760 cm^-1等波长段。结论 红外光谱技术对于鉴定体内外精液（斑）留存时间具有一定的可行性，体内外精液（斑）的降解趋势有所不同。更多还原

【Abstract】 Objective To identify the retention time of semen（stains） inside and outside the cadaver using Fourier Transform Infrared（FTIR） spectroscopy and chemometrics. Methods A simulated environment inside the porcine vagina was used to retain fresh semen for 0 ～ 168 hours inside and outside the body. Infrared spectroscopy data were collected, and chemometric methods including Principal Components Analysis（PCA） and Partial Least Squares（PLS） regression models were established. Results The infrared spectrum peak positions of semen（stains） inside and outside the body did not show significant changes over time. The PCA results showed good clustering relationships, and the predicted deviation value in the PLS regression model was 15.786 3 hours, with an R² value of 0.922 4. The characteristic wavenumbers selected by the CARS algorithm were in the wavelength ranges of 1 000 ～ 1 180 cm^-1, 1 430 ～ 1 480 cm^-1, and 1 700 ～ 1 760 cm^-1.Conclusion Fourier Transform Infrared spectroscopy has the potential to identify the retention time of semen（stains） inside and outside the body, and there are differences in the degradation trends of semen（stains） inside and outside the body.更多还原