【摘要】 目的 分析HMGB1基因在子宫腺肌病上皮间质转化过程中的表达，并探讨其与β-catenin的相关性，以探究子宫腺肌病的发病机制，为该病的临床治疗提供新的理论基础。方法 选取我院收治的行子宫切除术的子宫腺肌病患者8例，取其在位内膜组织样本为在位组，取其异位内膜组织样本为异位组，并以同期手术病理证实正常子宫内膜的子宫肌瘤患者为对照组，采用qRT-PCR检测HMGB1基因的表达水平；Western blot法检测HMGB1、E-cadherin、Vimentin、β-catenin的蛋白表达水平，并分析表达的相关性。结果 与对照组相比，在位组及异位组中HMGB1 mRNA和蛋白、Vimentin蛋白、β-catenin蛋白的表达水平升高，E-cadherin蛋白表达水平降低(P<0.05);与在位组相比，异位组中表达HMGB1 mRNA和蛋白、Vimentin蛋白的表达水平增加，E-cadherin蛋白表达水平下降(P<0.05);但β-catenin蛋白的表达与异位组和在位组比较，差异无统计学意义(P>0.05)。在相关性分析中，子宫腺肌病在位组、异位组的HMGB1蛋白表达与E-cadherin呈负相关(P<0.05),与Vimentin、β-catenin呈正相关(P<0.05)。结论 HMGB1基因可促进子宫腺肌病上皮间质转化的发生，与激活Wnt/β-catenin信号通路相关。更多还原

【Abstract】 Objective To analyze the function of HMGB1(high mobility group box-1) gene on epithelial mesenchymal transition( EMT) in adenomyosis, and explore its correlation with β-catenin. To study the mechanism of adenomyosis and provide a new theoretical basis for its clinical treatment.Methods Eight patients of adenomyosis underwent hysterectomy in Fuzhou Second Hospital were selected.The eutopic endometrial tissue samples were taken as the eutopic group, and the ectopic endometrial tissue samples were taken as the ectopic group.The normal endometrial tissue samples from 8 cases of hysteromyoma underwent operation confirmed by pathological examination were selected as control group.The mRNA expression of HMGB1 was detected by qRT-PCR.And the protein expression of HMGB1, E-cadherin, Vimentin and β-catenin were detected by Western blot.And the correlation was analyzed by correlation analysis.Results Compared with those in control group, the mRNA expression levels of HMGB1 and the protein expression levels of HMGB1, Vimentin, β-catenin in eutopic group and ectopic group were significantly higher, while the protein expression level of E-cadherin in them was significantly lower(P<0.05).Compared with those in eutopic group, the mRNA and protein expression levels of HMGB1 and the protein expression levels of Vimentin in ectopic group were obviously higher, but the protein expression level of E-cadherin in ectopic group was obviously lower(P<0.05). However the difference of the protein expression levels of β-catenin between eutopic group and ectopic group was not statistically significant(P>0.05).In the study of correlation analysis, the protein expression levels of HMGB1 in eutopic group and ectopic group were positive correlated with those of Vimentin、β-catenin(P<0.05), which were negative correlated with those of E-cadherin(P<0.05).Conclusion HMGB1 maybe promote the process of EMT of adenomyosis, and is correlated with the simulation of the Wnt/β-catenin signaling pathway.更多还原