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不同投饵率对黑鲷及其杂交子二代幼鱼影响差异

Effects of different feeding rates on the hybrid porgy F2 of Acanthopagrus schlegelii♀ × Pagrus major ♂ and A. schlegelii

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【作者】 孙瑞健仇玉燕杨志强徐大凤肖李霞秦亚丽倪可雯周堂建陈淑吟

【Author】 SUN Ruijian;QIU Yuyan;YANG Zhiqiang;XU Dafeng;XIAO Lixia;QIN Yali;NI Kewen;ZHOU Tangjian;CHEN Shuyin;Marine Fisheries Research Institute of Jiangsu Province;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University;Freshwater Fisheries Research Institute of Jiangsu Province;Qidong Fishery Technology Extension Station;

【通讯作者】 陈淑吟;

【机构】 江苏省海洋水产研究所上海海洋大学水产科学国家级实验教学示范中心江苏省淡水水产研究所江苏省启东市渔业技术推广站

【摘要】 为探讨黑鲷(Acanthopagrus schlegelii)♀×真鲷(Pagrus major)♂杂交子二代(杂交鲷)与黑鲷幼鱼适宜的投饵率,实验设计了2.5%、4.0%、5.5%及7.0%等4个投饵率,并比较了两种实验鱼在不同投饵率下的生长性能、生长相关基因表达及消化酶活性等方面的差异表现。结果显示:随着投饵率升高,杂交鲷及黑鲷的末体质量(FBW)、增重率(WGR)、特定生长率(SGR)及类胰岛素生长因子(igf1基因)表达量均先升高后降低,且4.0%组显著高于其他3组(P<0.05);生长激素受体(ghr基因)表达量先降低后升高,4.0%组显著低于2.5%组(P<0.05),但与其他2组无显著差异(P>0.05);当投饵率升至4.0%时,脂肪酶活性显著降低(P<0.05);继续提高投饵率至7.0%,肝体指数、成活率及胃蛋白酶活性均显著降低(P<0.05);投饵率对两种幼鱼的体质量变异系数(CV)及饵料系数(FCR)等无显著影响(P>0.05);当投饵率为4.0%和5.5%时,杂交鲷的FBW、WGR、SGR、胃蛋白酶活性及igf1基因表达量均显著高于黑鲷(P<0.05)。研究结果表明,杂交鲷及黑鲷的幼鱼适宜投饵率为4.0%,且杂交鲷的生长性能及消化能力均优于黑鲷。

【Abstract】 In order to study the optimal feeding rate between the hybrid porgy(HF2) of Acanthopagrus schlegelii♀(black porgy) ×Pagrus major♂(red porgy) and black porgy, four feeding rates of 2.5%, 4.0%, 5.5% and 7.0% were designed to compare the differences in growth performance, growth related gene expression and digestive enzyme activity between the two kinds of fish under different feeding rates.The results showed that the final body weight(FBW), weight gain rate(WGR), specific growth rate(SGR) and igf1 gene expression levels of the HF2 and black porgy increased firstly and then decreased, and the 4.0% group was significantly higher than the other three groups(P<0.05); the expression of ghr gene in the 4.0% group was significantly lower than the 2.5% group, and there was no significant difference between the other two groups(P>0.05) when the feeding rate increased; the lipase activity decreased significantly while the feeding rate increased to 4.0%(P<0.05); and hepatosomatic index(HSI), survival rate(SR) and pepsin activity decreased significantly while the feeding rate was increased to 7.0%(P<0.05).Feeding rate had no significant effect on coefficient of variation and feed conversion ratio of the two fishes(P>0.05).FBW, WGR, SGR, pepsin activities and igf1 gene expression of the HF2 in the 4.0% and 5.5% groups were significantly higher than those of black porgy(P<0.05).The results revealed that the optimal feeding rates of the HF2 and black porgy was 4.0%, and the growth performance and digestive capacity of the HF2 were better than that of black porgy.

【基金】 江苏省种业振兴“揭榜挂帅”项目(JBGS[2021]034);江苏省农业重大新品种创制项目(PZC20-1744)
  • 【文献出处】 渔业研究 ,Journal of Fisheries Research , 编辑部邮箱 ,2023年02期
  • 【分类号】S965.231
  • 【下载频次】14
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