【摘要】 为提高BDE-47好氧降解菌的降解效率和环境适应性，作者以实验室前期研究的BDE-47好氧降解菌GB-2为供体菌，以AntABC基因簇为目的基因，使用从电子垃圾污染的土壤中筛选的土著优势菌WT-G为受体菌，构建了一株基因工程菌，并置于BDE-47污染土壤中开展模拟降解试验。结果表明：BDE-47好氧降解基因工程菌可稳定遗传，传代30代的菌株降解率可稳定保持在72%～76%之间。在100μg/L的BDE-47中，基因工程菌的BDE-47降解率（76.78%）高于供体菌GB-2(70.17%)；在1 500 ng/g的BDE-47污染土壤中，基因工程菌的BDE-47降解率（47.15%）也高于供体菌GB-2(41.98%)。BDE-47好氧降解基因工程菌的降解效果优于供体菌GB-2。BDE-47好氧降解基因工程菌降解能力较稳定，且对污染环境具有较好适应性。更多还原

【Abstract】 To improve the degradation efficiency and environmental adaptability of BDE-47 aerobic degrading bacteria, a genetically engineered bacterium was constructed using the BDE-47 aerobic degrading bacteria GB-2 studied in the laboratory as the donor bacteria and the AntABC gene cluster as the target gene, using the indigenous dominant bacteria WT-G screened from the soil contaminated by e-waste as the recipient bacteria. Simulated degradation test placed in BDE-47 contaminated soil. The results showed that BDE-47 aerobically degradable genetically engineered bacteria could be stably inherited, the degradation rate of the strain could be stably maintained between 72%～76% after 30 generations. In 100 μ g/L of BDE-47, the degradation rate of BDE-47 in genetically engineered bacteria(76.78%) was higher than that of donor bacteria GB-2(70.17%). In BDE-47 contaminated soil at 1 500 ng/g, the BDE-47 degradation rate(47.15%) of genetically engineered bacteria was also higher than that of donor bacteria GB-2(41.98%). The degradation effect of BDE-47 aerobically degradable genetically engineered bacteria was better than that of donor bacteria GB-2. BDE-47 aerobic degradation genetic engineering bacteria have stable degradation ability and good adaptability to polluted environment.更多还原