【摘要】 文章从冬季白浪河河口水体中分离筛选出1株耐盐耐低温的异养硝化-好氧反硝化菌WD0301，进行了形态观察及16S rDNA序列分析。研究了初始pH、C/N，转速、温度、盐度对WD0301异养硝化、好氧反硝化特性的影响。并进一步研究了菌剂WD0301实验室人工湿地系统对生物脱氮的增强作用。结果表明：该菌株为不动杆菌(Acinetobacter sp.)，发挥最佳异养硝化、好氧反硝化性能的最佳条件为pH 7.0、C/N 15～20、转速180 r/min、温度30℃。菌株均能在以NH4Cl、KNO3为唯一氮源的培养基中生长，并且36 h内NH4+-N、NO3--N去除率均达到95%以上。菌株可在盐度为40 g/L（以NaCl计）生长并且24 h内氨态氮去除率和硝态氮去除率分别为70.9%、52.2%；在15℃条件下24 h内氨态氮去除率和硝态氮去除率分别为46.4%、53.6%。在处理海水养殖尾水的人工湿地里加入菌株WD0301，其氨态氮降解效率较未强化人工湿地提高了16.9%。说明菌株WD0301具有良好的异养硝化性能，在生物强化人工湿地处理海水养殖尾水试验中具有较好的处理效果，可为菌剂应用与生物强化脱氮技术提供理论支持。更多还原

【Abstract】 An efficient heterotrophic nitrification-aerobic denitrifies bacteria species that salt-tolerant and cold-tolerant was isolated from the Bailang River estuary water. The strain WD0301 was analyzed by morphological and 16S r DNA sequence.The effects of temperature, salinity, initial pH, C/N and rotation speed on the nitrogen removal characteristics of WD03010were investigated. WD0301 inoculate were enriched and applied to a constructed wetland system to investigate their bioaugmentation effect on nitrogen removal. The results showed that WD0301 is belong to Acinetobacter sp. Efficient heterotrophic nitrification-aerobic denitrification of strain WD0301 occurred with pH of 7. 0, C/N ratio of 15～20, a rotating speed of 180 r/min and temperature of 30 ℃. WD0301 was able to grow in the culture medium with NH4Cl or KNO3as the sole sources of nitrogen, and the removal rate of NH4+-N and NO3--N reached 95% within 36 h. The strain can grow at a salinity of 40 g/L(in term of NaCl), ammonia and nitrate removal rate were at 70.9% and 52.2% in 24 h. At 15 ℃, the ammonia and nitrate removal rates were 46.4% and 53.6% in 24 h respectively. When strain WD0301 was added to the constructed wetland treated with the wastewater of sea culture, NH4+removal rate was increased by 16.9% compared with the unenhanced wetland nitrogen removal rate. It was indicated that strain WD0301 could remove nitrogen through heterotrophic nitrification-aerobic denitrification quickly and effectively. It has good treatment effect in the experiment of bioenhancer constructed wetland treating the tail water of mariculture, providing theoretical support for bacterial agent application and bioenhancing nitrogen removal technology.更多还原