【摘要】 镁离子转运蛋白（Magnesium transporter,MGT）是植物体内镁离子吸收运输的重要转运子。为系统研究番木瓜MGT家族（CpMGTs）特征，鉴定了基因家族成员并分析其蛋白特征和基因转录模式。结果表明番木瓜基因组含5个CpMGT家族成员（CpMGTa-CpMGTe），氨基酸数387～491个，分子量43.95～54.84 KD，定位于细胞核、细胞质、叶绿体、过氧化物酶体等亚细胞结构。基于转录组测序，发现CpMGTs的表达具有组织特异性，成熟叶、幼花与根器官中优势表达的CpMGT不同。根、花器官中CpMGTc表达具有性别差异性；CpMGTb和CpMGTd跨季节转录变化在雌雄番木瓜幼花中也存在特定差别。这些发现揭示CpMGTs表达模式及性别差异性表达特征，为研究基因功能奠定基础。更多还原

【Abstract】 Magnesium Transporter(MGT) was charactered as an important transporter of magnesium absorption and transportation in plant. To investigate characteristics of papaya(Carica papaya) magnesium transporters(CpMGTs),their gene family members were identified,and their protein characteristics and gene transcriptional patterns were analyzed. The results showed that five CpMGT members(CpMGTa-CpMGTe) with 387-491 amino acids and molecular weight of 43.95-54.84 KD,which were predicted to be located in nucleus,cytoplasm,chloroplast,peroxisome and other subcellular strucures,existde in papaya genome. According to the transcriptomic sequencing and analyses,tissue-specific expressional patterns of CpMGTs were observed in different organs,and the predominantly expressed CpMGT members were distinct among mature leaves,young flowers and roots. CpMGTs transcriptional profiles had showed specific sexual differences,i.e. CpMGTc displayed sex-biased gene expression in roots and flowers,while CpMGTb and CpMGTd showed their seasonal change tendencies of gene transcription in distinct manner between male and female papayas. All these findings charactered the expressional patterns and the special sex bias in CpMGTs transcription,laying the foundation for functional study of CpMGTs.更多还原