【摘要】 为克服测试时环境变化对荧光检测的影响，我们设计合成了比率型荧光探针，利用双波长的荧光发射来有效消除背景干扰。本文以邻苯二胺、四硼酸钠和1-甲基-3-烯丙基咪唑溴盐为反应前体，利用一步水热法合成了基于碳点双发射的荧光探针L-CDs，并实现了对铜离子（Cu2+）的双信号响应。L-CDs表现出荧光双发射现象，当激发波长为380 nm时，呈现出440 nm和570 nm的双发射峰。Cu²⁺可使探针在440 nm处的荧光发射强度减弱，同时570 nm的荧光发射峰增强。Cu²⁺浓度在0.04～0.244 mmol/L范围内时，与荧光比率信号（F570/F440）表现出良好的线性相关，检出限（LOD）为0.6μmol/L。所构建的荧光探针可用于实际水样中Cu²⁺的检测，回收率为99.4%～101.8%。更多还原

【Abstract】 In order to overcome the influence of environmental change on fluorescence detection, a ratio fluorescence probe was designed and prepared, and dual-wavelength fluorescence emission was used to effectively eliminate background interference. Using o-phenylenediamine, sodium tetraborate and 1-methyl-3-allyl imidazolium bromide as precursors, a one-step hydrothermal method has been developed to synthesize a ratio fluorescent probe based on carbon dots（L-CDs）, and to achieve a double signal response to heavy metal ions(Cu²⁺). L-CDs exhibited fluorescence double emission phenomenon, and showed double emission peaks at 440 nm and 570 nm when the excitation wavelength was 380 nm. The addition of Cu²⁺ can reduce the fluorescence emission intensity at 440 nm and increase the fluorescence emission peak value at 570 nm. The concentration of Cu²⁺ in the range of 0. 04-0. 244 mmol/L showed a good linear correlation with the fluorescence ratio signal（F570/F440）, and the detection limit（LOD） was 0. 6 μmol/L. The fluorescence probe can be used for the detection of Cu²⁺ in actual water samples with the recovery of 99. 4%-101. 4%.更多还原