【摘要】 试验旨在探索辣蓼黄酮提取物对南美白对虾免疫功能的调节及其作用机制。将1 000尾南美白对虾随机平均分为空白对照组，黄芪对照组，辣蓼黄酮提取物高（8.0 g/kg饲料）、中（4.0 g/kg饲料）、低（2.0 g/kg饲料）剂量试验组，连续给药35 d，于第17、35天测定对虾体长、体重，肝胰腺指数、血细胞分类计数、免疫相关酶活性和含量及肝胰腺组织中免疫相关因子的mRNA表达水平。结果表明，用药后第17天，低剂量组南美白对虾终末体长、体重和增重率与空白对照组相比差异不显著（P>0.05）。第35天时，低中高剂量组的iNOS均极显著高于空白对照组（P<0.01）；中剂量试验组南美白对虾血液中ACP和TP活性与空白对照组比较差异极显著（P<0.01），中剂量组南美白对虾终末体长、体重和增重率均极显著高于空白对照组（P<0.01）；低、中、高剂量组血液中颗粒细胞比例极显著高于空白对照组（P<0.01）；血液中TP、SOD、T-AOC、iNOS、ACP、AKP等免疫相关酶活性和含量以及肝胰腺组织中ProPO、PE、LGBP等免疫相关因子的mRNA表达水平均呈不同程度升高。说明辣蓼黄酮提取物对南美白对虾具有促生长、抗氧化和免疫调节作用，其作用机制可能是增加血液中免疫细胞数量和升高相关酶活性及调节免疫相关因子的基因表达水平。更多还原

【Abstract】 The aim of this study was to find out the effects of flavonoid extract of Polygonum hydropiper L.(FPH) on immune function of penaeus vannamei and its mechanism.A total of 1 000 penaeus vannamei were randomly divided into 5 groups,which were blank control group,drug control group,high FPH group (8.0 g/kg feed),medium FPH group (4.0 g/kg feed),low FPH group (2.0 g/kg feed).The drugs were administered continuously for 35 days.The body length,body weight,hepatopancreas index,proportion of blood cells and the activities and contents of immune-related enzymes were measured at the17th and 35th day respectively.In addition,the mRNA expression levels of immune-related factors in hepatopcreatic tissue were also measured.The results showed that there were no significant differences in the final body length,body weight and weight gain rate between the low do se group and the blank control group on the 17 th day after treatment (P>0.05).After 35 days of drug administration,iNOS in the low,medium and high FPH groups was extremely higher than that in the blank control group (P<0.01).The activities of ACP and TP in the blood of Penaeus vannamei in the middle FPH groups were extremely different from those in the blank control group (P<0.01),the final body length,weight and weight gain rate of Penaeus vannamei in the middle dose group were significantly higher than those in the blank control group (P<0.01);the proportion of granulosa cells in the blood of the low,medium and high dose groups was extremely higher than that in blank controls (P<0.01).The activities and contents of immune-related enzymes such as TP,SOD,T-AOC,i NOS,ACP and AKP in blood and the m RNA expression level of immune-related factors such as Pro PO,PE and LGBP in hepatopancreatic tissues were increased in varying degrees.In brief,FPH showed growth-promoting,antioxidant and immunomodulatory effects in Penaeus vannamei.The mechanism might be to improve the immune function of Penaeus vannamei by increasing the number of immune cells,increasing the activity of related enzymes and regulating the gene expression level of immune-related factors.更多还原