【摘要】 目的 对2021年北京市急性腹泻患儿A组双埃可病毒(parechovirus A,PeV-A)的感染状况及基因型别进行研究。方法 2021年1～12月，每月随机抽取北京市腹泻监测某哨点医院门诊就诊的<60月龄急性腹泻患儿粪便标本。提取病毒核酸，通过实时荧光RT-PCR检测PeV-A。用巢式RT-PCR扩增PeV-A的VP3/VP1连接区，通过测序和NCBI BLAST程序比对确定PeV-A基因型别；用实时荧光PCR对A组轮状病毒、诺如病毒、肠道腺病毒、星状病毒和札如病毒进行检测，分析混合感染状况。利用生物信息学及统计软件分别完成进化和统计分析。结果 198例粪便标本中，PeV-A阳性11例，检出率为5.56%(11/198)。其中，2例混合肠道腺病毒感染。81.82%(9/11)的PeV-A阳性患儿<24月龄。秋季检出率最高，为12.50%(7/56)。90.91%(10/11)的PeV-A感染集中在夏秋两季。11例阳性标本中有9例测序成功，其中，7例为PeV-A1B型，2例为PeV-A3型。结论 2021年北京市急性腹泻患儿感染PeV-A共检出PeV-A1B和PeV-A3两种型别，2岁以内婴幼儿是PeV-A感染的好发人群。腹泻患儿感染PeV-A主要集中在夏秋两季。更多还原

【Abstract】 Objective To investigate the epidemiological chatacteristics and genotypes of parechovirus A(PeV-A) from children with acute diarrhea in Beijing in 2021. Methods Fecal samples were randomly collected from outpatient children under 60 months with acute diarrhea in a sentinel hospital in Beijing from January to December of 2021.RNA was extracted and detected for PeV-A by real-time RT-PCR.Nested RT-PCR was performed to amplify the VP3/VP1 conjunction region.PeV-A genotypes were determined based on sequencing and NCBI BLAST.Group A rotavirus, norovirus, enteric adenovirus, astrovirus and sapovirus were also detected for co-infection analysis.Phylogenetic and statistical analyses were performed using bioinformatics and statistical software. Results Of the 198 stool samples, 11 were positive for PeV-A,with a detection rate of 5.56%(11/198).Among them, 2 cases were co-infected with enteric adenovirus.81.82%(9/11) of PeV-A infected cases were under 24 months.The highest detection rate was observed in fall, which was 12.50%(7/56).90.91%(10/11)of PeV-A infection occurred in summer and fall.Among the 11 PeV-A isolates, 9 were sequenced successfully, of which 7 belonged to PeV-A1B genotype and 2 belonged to PeV-A3 genotype. Conclusion PeV-A1B and PeV-A3 are identified in children with acute gastroenteritis in Beijing in 2021.Infants and young children under 2 years old are the high-risk population for PeV-A infection.Most infections occur in summer and fall.更多还原