【摘要】 叶绿素荧光成像作为一种高效的植物光合作用和生理状态信息获取手段，常被应用于智慧农业信息感知等众多领域。现阶段的大尺度叶绿素荧光成像技术缺少与微观的叶片级叶绿素荧光成像之间的机制上联系，忽略成像角度与叶片叶龄对叶绿素荧光成像产生的影响，限制了其在大尺度农业智能评估与决策中应发挥的作用。针对上述问题，设计了一套可多角度成像的叶绿素荧光采集设备，用以探究叶绿素荧光成像与成像角度和叶片叶龄之间存在的联系，并利用绿萝、樟树和大叶黄杨这三种分别代表藤本、乔木和灌木的植物共计60片叶子进行成像实验，分析荧光强度、Fv/Fm和Rfd三个典型荧光指标。实验结果表明成像角度对叶绿素荧光成像存在显著影响，成像角度的增大会导致荧光参数值的减小，对不同种类植物的影响也不同。叶片的叶龄也会影响叶绿素荧光成像，成长期叶片的成像参数指标优于成熟期叶片。更多还原

【Abstract】 Chlorophyll fluorescence imaging, as an efficient means of obtaining plant photosynthesis and physiological state information, is often used in many fields such as smart agricultural information perception. The current large-scale chlorophyll fluorescence imaging technology lacks the mechanistic connection with the microscopic leaf-level chlorophyll fluorescence imaging,ignoring the impact of imaging angle and leaf age on chlorophyll fluorescence imaging, which limits its application in large-scale agricultural intelligence assessment and role in decision-making. Aiming at the above problems, this paper designed a set of chlorophyll fluorescence acquisition equipment capable of multi-angle imaging to explore the relationship between chlorophyll fluorescence imaging, imaging angle and leaf age. A total of 60 leaves of epipremnum aureum, camphor and euonymus, representing vines, trees and shrubs were subjected to imaging experiments, and the fluorescence intensity, Fv/Fmand Rfd three typical fluorescence indicators were analyzed. The experimental results show that the imaging angle has a significant impact on the chlorophyll fluorescence imaging, and the increase of the imaging angle will lead to the decrease of the fluorescence parameter value, and the effect on different types of plants is also different. Leaf age also affects chlorophyll fluorescence imaging, and the imaging parameters of growing leaves are better than those of mature leaves.更多还原