【摘要】 为了解A、B两种商用猪繁殖与呼吸综合征减毒活疫苗免疫猪后病毒血症及抗体水平的差异，在后备母猪场随机选择100头35日龄后备母猪，分为A、B两组，分组后猪混养14 d。试验猪50日龄时，对A组50头后备母猪免疫A疫苗(1头份/头),B组50头后备母猪免疫B疫苗(1头份/头),疫苗免疫后0、1、2、3、5、7、10、14、21、28、35 d各组猪随机采集血样10份，荧光定量PCR检测猪繁殖与呼吸综合征病毒载量，ELISA检测猪繁殖与呼吸综合征病毒抗体。结果显示，B疫苗免疫后，试验猪的猪繁殖与呼吸综合征病毒病毒血症期持续时间更久，持续至免疫后35 d; A疫苗免疫后，试验猪的猪繁殖与呼吸综合征病毒载量更高；B疫苗免疫后试验猪的N蛋白抗体水平变化趋势更稳定；B疫苗免疫后试验猪的PRRSV GP蛋白抗体水平更高。试验结果表明B疫苗的免疫效果优于A疫苗。更多还原

【Abstract】 In order to understand the differences in viremia and antibody levels in pigs immunized with live attenuated commercial porcine reproductive and respiratory syndrome vaccines A and B,100 35 day old reserve sows were randomly selected from the reserve sow farm and divided into groups A and B.After grouping, the pigs were mixed bred for 14 days.At the age of 50 days, 50 reserve sows in group A were immunized with vaccine A,and 50 reserve sows in group B were immunized with vaccine B(1 portion per head).On days 0,1,2,3,5,7,10,14,21,28,and 35 of vaccine immunization, 10 samples of sera were randomly collected and isolated from pigs in each experimental group.The viral load of porcine reproductive and respiratory syndrome was detected by fluorescence quantitative PCR,and the antibody to porcine reproductive and respiratory syndrome virus was detected by ELISA.The results showed that: After immunization with B vaccine, the duration of the experimental porcine reproductive and respiratory syndrome viremia period was longer, lasting up to 35 days; After immunization with vaccine A,the experimental porcine reproductive and respiratory syndrome virus load was higher; After immunization with B vaccine, the variation trend of N-protein antibody level in pigs was more stable; After immunization with B vaccine, the level of GP protein antibody in pigs was higher.This indicates that the immune effect of vaccine B is better than that of vaccine A.更多还原