【摘要】 探究黄芩苷对H6N6亚型禽流感病毒诱导小鼠肺脏致病损伤的作用。选用40只昆明小鼠随机分为4组，即对照组、模型组、黄芩苷治疗组、3-MA抑制剂组。通过HE染色观察肺组织病理形态学变化，ELISA法检测血清中IL-1β、IL-2、IL-6及TNF-α的含量，透射电镜检测小鼠肺脏中自噬体的水平，Western blot检测肺组织LC3、Beclin-1及ATG7蛋白表达水平，免疫荧光法检测肺组织LC3蛋白表达水平，免疫组化法检测肺组织LC3、Beclin-1及ATG5蛋白表达水平，RT-qPCR检测肺组织自噬关键基因LC3、Beclin-1、ATG3、ATG5、ATG7及ATG12 mRNA转录水平。结果显示，经黄芩苷治疗后能显著缓解H6N6亚型禽流感病毒对小鼠肺组织的损伤，血清中IL-1β、IL-2、IL-6及TNF-α含量显著下降(P<0.01),肺组织中自噬体数量减少，LC3、Beclin-1、ATG5及ATG7蛋白表达水平显著下降(P<0.01),肺组织中LC3、Beclin-1、ATG3、ATG5、ATG7及ATG12 mRNA转录水平显著下降(P<0.01)。结果表明，黄芩苷能减轻H6N6亚型禽流感病毒诱导小鼠肺脏炎症损伤，同时下调自噬关键因子及自噬体的水平，提示其抗小鼠肺损伤机制可能与调控细胞自噬有关。更多还原

【Abstract】 To investigate the effect of baicalin on lung injury induced by H6N6 avian influenza virus in mice, forty Kunming mice were randomly divided into 4 groups: control group, model group, baicalin treatment group and 3-MA inhibitor group.The histopathological lesions of lung tissues were observed by HE staining.The levels of IL-1β,IL-2,IL-6 and TNF-α in sera were detected by ELISA.The level of autophagosome in lungs of mice was detected by transmission electron microscopy.The protein expressions of LC3,Beclin-1 and ATG7 in lung tissue were detected by Western blot.Immunofluorescence was used to detect the expression of LC3 protein in lung tissue.The protein expression levels of LC3,Beclin-1 and ATG5 in lung tissue were detected by immunohistochemistry.RT-qPCR was used to detect the mRNA transcription levels of autophagy key genes LC3,Beclin-1,ATG3,ATG5,ATG7 and ATG12.The results showed that baicalin could significantly alleviate the injury of lung tissue induced by H6N6 avian influenza virus in mice.Serum levels of IL-1β,IL-2,IL-6 and TNF-α were significantly decreased(P<0.01); The number of autophagosomes in lung tissue decreased; The protein expression levels of LC3,Beclin-1,ATG5 and ATG7 were significantly decreased(P<0.01); The mRNA transcription levels of LC3,Beclin-1,ATG3,ATG5,ATG7 and ATG12 in lung tissue decreased significantly(P<0.01).The results showed that baicalin could reduce lung inflammatory injury induced by H6N6 avian influenza virus in mice, and down-regulate the levels of autophagy key factors and autophagosomes, suggesting that the mechanism of baicalin against lung injury in mice may be related to the regulation of autophagy.更多还原