【摘要】 为了解家鼠在PRRSV传播中的特点和作用，进而初步评估环境生物传播PRRSV的潜在风险。捕捉猪场家鼠，收集鼠肠道粪便，提取RNA后采用RT-PCR技术扩增PRRSV的NSP2、ORF5、NSP9、NSP10基因，然后与来源于同一猪场猪源PRRSV以及其他PRRSV代表株相应的基因进行生物学信息比较，并结合Swiss-Model和ProtScale等软件对其中主要差异基因编码蛋白的三维结构及理化特点做进一步分析。结果显示，鼠肠道粪便中的PRRSV NSP2、ORF5、NSP9和NSP10基因与来源同一猪场感染猪样本中相应的PRRSV基因同属于美洲型毒株，且与高致病性PRRSV GD株亲缘关系最近，但鼠源和猪源PRRSV的NSP2、ORF5、NSP9、NSP10之间有多个氨基酸的替换和缺失。尤其是主要差异基因NSP10所编码的NSP10蛋白，三维结构分析结果显示二者具有较高的相似性，主要由疏水性氨基酸组成，但它们的无规则卷曲区和腺苷三磷酸酶结构域的ATP结合位点在氨基酸组成存在差异。研究表明鼠肠道粪便中PRRSV NSP9、NSP10、NSP2和ORF5基因和来源于同一猪场感染猪样本中的PRRSV,与其他PRRSV代表株之间存在不同程度的差异，这些差异是否会影响PRRSV生物学特性需要进一步验证。更多还原

【Abstract】 To better understand the characteristics and role of vector animals in PRRSV transmission, and then preliminarily evaluate the potential risk of environmental biological transmission of PRRSV.Stool samples were collected from house mice captured from pig farms in this study.After RNA was extracted, the NSP2,ORF5,NSP9 and NSP10 genes of PRRSV were amplified by RT-PCR,and then the biological information was compared with the corresponding genes of PRRSV from the same pig farm and other representative PRRSV strains.Meanwhile, combining with the software of Swiss-Model and ProtScale, the three-dimensional structure and the physical and chemical characteristics of the proteins encoded by the main differential genes were analyzed.The results showed that the PRRSV NSP2,ORF5,NSP9 and NSP10 genes originated in intestinal feces of house mice and the corresponding genes in pig samples infected with PRRSV from the same pig farm belonged to the American strain, and were closely related to the highly pathogenic PRRSV GD strain.However, there were many amino acid substitutions and deletions among NSP2,ORF5,NSP9 and NSP10 of PRRSV from mouse and pig.In particular, the three-dimensional structure analysis of NSP10 protein encoded by the main differential gene NSP10 shows that they have high similarity, which was mainly composed of hydrophobic amino acids, but their irregular curl region and ATP binding site of adenosine triphosphatase domain were different in amino acid composition.In conclusion, there are some differences in NSP9,ORF10,NSP2 and NSP5 genes of PRRSV from mouseintestinal feces, the matched pig sample infected with PRRSV and the other representative PRRSV strains.Whether these differences will affect the biological characteristics of PRRSV needs further verification.更多还原