【摘要】 本试验旨在探究罗汉果总黄酮对慢性睡眠剥夺（CSD）小鼠抗氧化能力及炎症反应的影响。将42只4周龄无特定病原体（SPF）级雄性ICR小鼠按体质量随机分为7组，每组6只，组间体质量差异不显著（P>0.05）。空白对照组不进行慢性睡眠剥夺，其他6组使用改良多水平台睡眠剥夺法进行为期30 d的慢性睡眠剥夺，以构建CSD模型。造模成功后灌胃4周，其中低、中、高浓度罗汉果总黄酮组（分别简称低、中、高酮组）分别灌胃30、60、120 mg/（kg·d）的罗汉果总黄酮（溶于0.5 mL的1%DMSO溶液），空白对照组与模型对照组灌胃0.5 mL的生理盐水，阳性对照组灌胃30 mg/（kg·d）的褪黑素（溶于0.5 mL的1%DMSO溶液），二甲基亚砜（DMSO）对照组灌胃0.5 mL的1%DMSO溶液。利用相应试剂盒测定CSD小鼠血清和大脑中总抗氧化能力（T-AOC）、丙二醛（MDA）含量、血红素氧合酶-1 (HO-1)活性、核因子-κB(NF-κB)和肿瘤坏死因子-α(TNF-α)含量，利用实时荧光定量PCR测定血清和大脑中核因子E2相关因子2(Nrf2)、HO-1、NF-κB、TNF-α mRNA相对表达量。结果显示：1）低、中、高酮组CSD小鼠大脑中T-AOC显著高于模型对照组（P<0.05），中、高酮组CSD小鼠大脑中HO-1活性显著高于模型对照组和DMSO对照组（P<0.05）。低、中、高酮组CSD小鼠血清中T-AOC、HO-1活性显著高于模型对照组和DMSO对照组（P<0.05），大脑和血清中MDA、NF-κB、TNF-α含量显著低于模型对照组和DMSO对照组（P<0.05）。2）低、中、高酮组CSD小鼠大脑和血清中HO-1 mRNA相对表达量显著高于模型对照组及DMSO对照组（P<0.05）；中、高酮组CSD小鼠大脑和血清中Nrf2 mRNA相对表达量显著高于模型对照组及DMSO对照组（P<0.05）；低、中、高酮组CSD小鼠大脑和血清中NF-κB、TNF-α mRNA相对表达量显著低于模型对照组及DMSO对照组（P<0.05）。综上所述，罗汉果总黄酮可提高CSD小鼠大脑、血清中抗氧化酶活性及相关基因mRNA相对表达量，降低大脑、血清中MDA与炎症因子含量及相关基因mRNA相对表达量，改善CSD小鼠的氧化应激及炎症反应。更多还原

【Abstract】 The aim of this experiment was to investigate the effects of total flavonoids of Siraitia grosvenorii on antioxidant ability and inflammatory responses in mice with chronic sleep deprivation(CSD). Forty-two 4-week-old specific-pathogen-free(SPF)grade male ICR mice were randomly divided into 7 groups of 6 mice each according to body mass,and the difference in body mass among groups was not significant(P>0.05).Blank control group did not undergo CSD,and the remaining 6 groups underwent CSD for 30 days to construct CSD model using a modified multilevel table sleep deprivation method. After successful establishing the model,mice were gavaged for 4 weeks. The low,medium and high concentrations of total flavonoids of Siraitia grosvenorii(hereinafter referred to as low-,medium-and high-flavonoids groups,respectively)were gavaged with 30,60 and 120 mg/（kg·d）of total flavonoids of Siraitia grosvenorii(solved in 0.5 mL 1% DMSO solution),respectively,the blank control group and the model control group were both gavaged with 0.5 mL normal saline,the positive control group was gavaged with 30 mg/（kg·d）of melatonin(solved in 0.5 mL1% DMSO solution),and the dimethyl sulfoxide(DMSO)control group was gavaged with 0.5 mL 1% DMSO solution. The total antioxidant capacity(T-AOC),malonic dialdehyde(MDA)content,heme oxygenase-1(HO-1)activity,nuclear factor kappa-B(NF-κB)and tumour necrosis factor-α(TNF-α)contents in the serum and brain of CSD mice were measured by using the corresponding kits. The mRNA relative expression levels of nuclear factor E2 related factor 2(Nrf2),HO-1,NF-κB and TNF-α in the serum and brain of CSD mice were determined by real-time fluorescence quantitative PCR. The results showed as follows:1)the T-AOC in the brain of CSD mice in the low-,medium-and high-flavonoids groups was significantly higher than that in the model control group(P<0.05),the HO-1 activity in the brain of CSD mice in the mediumand high-flavonoids groups was significantly higher than that in the model control group and DMSO control group(P<0.05). The T-AOC and HO-1 activity in the serum of CSD mice in the low-,medium-and highflavonoids groups were significantly higher than that in the model control group and DMSO control group(P<0.05),while the MDA,NF-κB and TNF-αcontents in the serum and brain of CSD mice were significantly lower than that in the model control group and DMSO control group(P<0.05). 2)The mRNA relative expression level of HO-1 in the brain and serum of CSD mice in the low-,medium-and high-flavonoids groups were significantly higher than those in the model control group and DMSO control group(P<0.05);the mRNA relative expression level of Nrf2 in the brain and serum of CSD mice in the medium-and high-flavonoids groups was significantly higher than that in the model control group and DMSO control group(P<0.05);the mRNA relative expression levels of NF-κB and TNF-α in the brain and serum of CSD mice in the low-,medium-and high-flavonoids groups were significantly lower than those in the model control group and DMSO control group(P<0.05). In conclusion,the total flavonoids of Siraitia grosvenorii can increase the activities of antioxidant enzyme and the mRNA relative expression levels of related genes in the brain and serum of CSD mice,decrease the contents of MDA and inflammatory factors and the mRNA relative expression levels of related genes in the brain and serum,and then improve the oxidative stress and inflammatory responses in CSD mice.[Chinese Journal of Animal Nutrition,2023,35(7):4668-4677]更多还原