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光合细菌和小球藻对石生苔藓种源扩繁的作用

Effects of Photosynthetic Bacteria and Chlorella vulgaris on Provenance Cultivation of Lithophytic Moss Crusts

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【作者】 王鹤鸣; 卜崇峰; 吴淑芳; 王春; 莫秋霞;

【Author】 WANG Heming;BU Chongfeng;WU Shufang;WANG Chun;MO Qiuxia;College of Water Resources and Architectural Engineering, Northwest A&F University;Institute of Soil and Water Conservation, Northwest A&F University;Institute of Soil and Water Conservation, CAS and MWR;College of Natural Resources and Environment, Northwest A&F University;

【通讯作者】 卜崇峰;

【机构】 西北农林科技大学水利与建筑工程学院; 西北农林科技大学水土保持研究所; 中国科学院水利部水土保持研究所; 西北农林科技大学资源与环境学院;

【摘要】 石生苔藓结皮可以从大气中吸收水分及养分,附着在岩石表面生长,具有促进矿物分化和植被演替等多种重要的生态功能,利用人工培育的苔藓结皮进行岩石工程创面生态修复具有广阔的市场前景,而针对特定地区的优势种探讨高效的种源扩繁技术则成为了首要任务。研究以秦岭北麓长势好、生物量大的石生匍匐型藓种羽枝青藓为研究材料,考虑沼泽红假单胞菌浓度(高浓度24 mL/L,低浓度12 ml/L,不添加)和小球藻浓度(高浓度250 mL/L,低浓度125 mL/L,不添加)开展双因素完全试验,观测人工气候箱条件下羽枝青藓的盖度、新发芽数和新发芽茎长,探究沼泽红假单胞菌和小球藻对羽枝青藓的作用效果、最适浓度及组合,为提高羽枝青藓扩繁效率提供科学依据。结果显示,(1)只添加低浓度沼泽红假单胞菌的处理羽枝青藓生长状况最优,相比于对照处理(不添加沼泽红假单胞菌和小球藻),可将盖度提高14.3%,新发芽数增加61.2%,新发芽茎长增长34.0%;(2)沼泽红假单胞菌浓度对羽枝青藓的盖度、新发芽数和新发芽茎长均有显著影响;(3)小球藻浓度对羽枝青藓各项生长指标均无显著影响,其与沼泽红假单胞菌的交互作用对羽枝青藓盖度有显著影响。研究表明,沼泽红假单胞菌对羽枝青藓的生长发育具有显著促进作用,在后期石生苔藓结皮种源扩繁实践中应加以考虑和借鉴。

【Abstract】 The lithophytic moss crusts can absorb water and nutrients from the atmosphere and grow on the rock surface, and it has a variety of important ecological functions, such as promoting mineral differentiation and vegetation succession. The use of artificially cultivated moss crusts for ecological restoration of rock engineering wounds has a broad market prospect, and it has become the primary task to explore efficient provenance cultivation techniques for the dominant species in specific areas. For this reason, in this study, a well-growth and high biomass stone creeping moss in the northern foot of Qinling Mountains, Brachythecium plumosum(Hedw.) B. S. G., was used as the research material, considering the concentration of Rhodopseudomonas palustris(24 mL/L, 12 mL/L, no addition) and Chlorella vulgaris concentration(250 mL/L, 125 mL/L, no addition) to carry out a two-factor complete experiment. The coverage, newly germinated number and newly germinated stem length of moss were observed under the condition of artificial climate box, and the effect, optimum concentration and combination of bacteria and algae were studied, so as to provide scientific basis for improving the efficiency of cultivation. The results showed,(1) only adding low concentration of bacteria was the best performance treatment of moss crusts growth. Compared with the control(no addition of bacteria or algae), it could increase the coverage by 14.3%, the number of new sprouts by 61.2%, and the length of new sprouts by 34.0%.(2) The concentration of bacteria had significant effects on the coverage, number of new sprouted moss and stem length of new sprouted moss.(3) The concentration of algae had no significant effect on the growth indexes of moss crusts, and the interaction with bacteria had a significant effect on the coverage. Rhodopseudomonas palustris could significantly promote the growth and development of Brachythecium plumosum(Hedw.) B. S. G., which should be considered and used for reference in the future provenance cultivation.

【基金】 国家自然科学基金面上项目(42371058);秦创原西部科技创业湾项目(2022-747);国家重点研发计划项目(2017YFC0504703);国家重点研发国际合作项目(2016YFE0203400-05)
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,2023年11期
  • 【分类号】Q945
  • 【下载频次】34
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