【摘要】 为探讨H~+-焦磷酸酶编码基因对甜菜磷吸收和抗性的影响，实现优良基因在甜菜基因工程中的利用，研究在甜菜中超表达拟南芥液泡膜H~+-焦磷酸酶编码基因AVP1,对转基因甜菜分析其耐低磷、耐盐性和抗旱性。结果显示，AVP1基因在甜菜植株的叶片和块根中表达，且在逆境胁迫下增强表达量响应胁迫；低磷处理条件下，转基因甜菜与野生型甜菜相比具有更高的含磷量，可提高甜菜对磷的吸收利用效率；干旱、盐胁迫处理条件下，AVP1基因在转基因甜菜中显著上升，在盐胁迫或干旱处理条件下，转基因植株的生长受抑程度相对较轻。随着盐和干旱胁迫的加剧，转基因植株体内MDA含量与野生型植株相比较低而脯氨酸含量显著增加，AVP1基因可通过减轻逆境对甜菜细胞膜的损伤及提高甜菜细胞的渗透调节能力，进而增强甜菜对高盐和干旱胁迫的抗性。更多还原

【Abstract】 In order to explore the effect of H~+-pyrophosphatase coding gene on phosphorus absorption and resistance of sugar beet, and to realize the utilization of excellent genes in sugar beet genetic engineering, AVP1 gene encoding vacuolar membrane H~+-pyrophosphatase of Arabidopsis thaliana was used to transform sugar beet, and the low phosphorus tolerance, salt tolerance and drought resistance of transgenic sugar beet were analyzed in this study. The results showed that AVP1 gene could be expressed in the roots and leaves of sugar beet plants, and the expression level increased under stress. Under the condition of controlling phosphorus supply, the phosphorus content of transgenic sugar beet was significantly higher than that of wild type. Under drought and salt stress, AVP1 gene increased significantly in transgenic sugar beet. Under 200 mmol/L NaCl treatment or 7 days after natural drought, the growth of sugar beet plants was inhibited, while the transgenic plants got less inhibited degree significantly. In addition, with the aggravation of salt and drought stress, compared with wild type plants, the MDA content of transgenic plants was lower and the proline content was significantly increased. AVP1 gene could reduce the damage of stress to the cell membrane of sugar beet, improve the osmotic adjustment ability of sugar beet cells, thereby enhancing the salt tolerance and drought resistance of sugar beet.更多还原