【摘要】 以L.gibbosa菌丝体总RNA反转录的cDNA为模板，通过RT-PCR克隆得到一个C2H2型锌指转录因子的cDNA序列。对该基因进行基因克隆、生物信息学分析，并利用实时荧光定量法对木屑处理下该基因的表达进行分析。通过保守结构域预测与结构分析发现该基因为裂指蛋白1类(SFP1)C2H2型锌指转录因子，具有2个C2H2家族的保守结构域，将该基因命名为Lg-sfp1。该基因CDS全长1 947 bp,编码一个由648个氨基酸组成的亲水性膜内蛋白，蛋白大小为68.27 ku。通过SWISS-MODEL预测的三级空间结构发现两个完整的C2H2结构。木屑处理下Lg-sfp1基因表达比无木屑处理下表达量低，推断木屑促进了L.gibbosa氧化应激反应，当氧化应激反应大量发生时，参与氧化应激反应负调控的sfp1基因表达受到抑制，为后续研究偏肿革裥菌sfp1基因在木材降解，特别是氧化应激反应中的功能提供了理论基础。更多还原

【Abstract】 A C2H2 zinc finger transcription factor was cloned by RT-PCR using cDNA reverse transcription of total RNA from L. gibbosa strain. The gene was cloned andanalyzedin bioinformatics, and the expression of the gene under sawdust treatment was analyzed by Quantitative Real-time PCR method. Through conservative domain prediction and structural analysis, the gene is a Split Zinc-finger protein 1(SFP1) C2H2 transcription factor with two C2H2 family conserved domains. The gene was named Lg-sfp1. The CDS length of the gene is 1 947 bp, encoding a hydrophilic membrane protein composed of 648 amino acids, with a protein size of 68.27 ku. Through thethree-dimensional structure predicted by SWISS-MODEL, two complete C2H2 structures were found. The expression level of Lg-sfp1 gene was lower under sawdust treatment than that without sawdust treatment, inferring that sawdust promoted oxidative stress response of Lg-sfp1 gene expression involved in negative regulation of oxidative stress response was inhibited when a large number of oxidative stress responses occurred.更多还原