【摘要】 为探究马唐对噁唑酰草胺抗性水平及产生抗药性的原因，采用整株生物测定法研究马唐对噁唑酰草胺抗性水平，对比高抗种群AD-10及敏感种群SD-1体内乙酰辅酶A羧化酶（ACCase）、细胞色素P450（P450s）、谷胱甘肽-S-转移酶（GSTs）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性差异。结果表明，供试马唐种群均对噁唑酰草胺产生不同程度的抗性，GR50值（有效成分，下同）在99.20～1 891.52 g·hm^-2；其中马唐种群AD-10、JD-1达高水平抗性，GR50值分别为1 891.52、1 309.88 g·hm^-2，抗性指数（RI）为68.38、47.36。高抗种群AD-10经噁唑酰草胺100μmol·L^-1处理后，ACCase酶活抑制率为45.94%，相对酶活性为67.80;P450s抑制剂马拉硫磷可提高AD-10对噁唑酰草胺的敏感性，处理后RI下降2.02。施用噁唑酰草胺120 g·hm^-2后，AD-10和SD-1体内的POD、CAT酶活性无差异；AD-10体内GSTs、SOD酶活性受抑制后逐渐恢复，SD-1不可恢复。可知，抗性马唐种群体内ACCase、GSTs和SOD活性对噁唑酰草胺敏感性降低是其产生抗药性的主要原因。更多还原

【Abstract】 In order to explore the resistance level of Digitaria sanguinalis to metamifop and the reasons for its resistance to metamifop, the whole-plant bioassay method was used to study the resistance level of D. sanguinalis to metamifop, and the activities of ACCase, P450s, GSTs, SOD, POD and CAT in high-resistant population AD-10 and sensitive population SD-1 were compared. The results showed that the tested populations of D. sanguinalis had different degrees of resistance to metamifop,with GR50（active ingredient, the same as the rest） ranging from 99.20 g · hm^-2to 1 891.52 g · hm^-2.Among them, the populations of AD-10 and JD-1 reached high level of resistance, with GR50of 1 891.52 and 1 309.88 g · hm^-2, and RI were 68.38 and 47.36. After treatment with 100 μmol · L-1metamifop, the inhibition rate of ACCase activity was 45.94%, and the relative enzyme activity was 67.80. The P450s inhibitor malathion increased the sensitivity of AD-10 to metamifop, and RI decreased by 2.02 after treatment. After application of 120 g · hm^-2metamifop, there was no significant difference in POD and CAT enzyme activities between AD-10and SD-1. The activities of GSTs and SOD in AD-10 could be gradually restored after inhibition, while SD-1 could not be restored. It concluded that the decreased sensitivity of ACCase, GSTs and SOD activities to metamifop in resistant D. sanguinalis population was the main reason for its resistance.更多还原