【摘要】 SBP-box作为植物特有转录因子家族，广泛参与植物生长发育调控与响应逆境胁迫。从芥菜全基因组中鉴定SBP-box基因家族，分析家族成员理化性质、序列特征、互作调控网络、进化关系及表达模式。结果表明，芥菜SBP-box共包含58个成员，均含有1个保守SBP结构域，启动子区域含有多种非生物胁迫响应与激素响应的顺式作用元件，BjuSBP-microRNA-靶基因构建复杂的调控网络，参与有机物生物合成、RNA代谢过程调控、花器官发育、营养相变化、细胞代谢调节等生物学过程。在低温胁迫下，BjuSBP1a、BjuSBP1b、BjuSBP1e、BjuSBP4l、BjuSBP4m等基因显著上调表达，在光胁迫下，大多数家族基因表达受抑制，其中BjuSBP1a、BjuSBP9d在两种胁迫下均上调表达，推断其在芥菜非生物胁迫应激反应中发挥功能作用，可作为培育芥菜抗逆新品种候选基因资源。更多还原

【Abstract】 As a plant-specific transcription factor family, SBP-box is widely involved in the regulation of plant growth and development and responding to abiotic stress. In this study, the SBP-box gene family was identified from the whole genome of Brassica juncea, and the physicochemical properties, sequence characteristics, interaction networks, evolutionary relationships, and expression patterns of the family members were analyzed. The analysis results showed that 58 members of the SBP-Box family were identified in Brassica juncea, all of which contained a conserved SBP domain,and the promoter region contained a variety of cis-acting elements for abiotic stress response and hormone response. BjuSBP-microRNA target genes formed complex regulatory networks and were involved in organic matter biosynthesis, regulation of RNA metabolism process, flower organs development, nutritional phase change, regulation of cell metabolism, and other biological processes.Under low temperature stress, the expression of BjuSBP1a, BjuSBP1b, BjuSBP1e, BjuSBP4l,BjuSBP4m and others was significantly up-regulated. While the expression of most of the family genes was inhibited under photostress, among them BjuSBP1a and BjuSBP9d were up-regulated under both stresses, suggesting that they played a functional role in the abiotic stress response of Brassica juncea,and could be used as candidate gene resources for breeding new varieties of stress resistance of Brassica juncea.更多还原