【摘要】 为探明西瓜（Citrullus lanatus）不同发育时期果实中葫芦素E积累机制，通过高效液相色谱法测定西瓜不同发育时期果实、叶片和茎蔓中葫芦素E含量，在葫芦科数据库中鉴定西瓜葫芦素E合成代谢关键基因，结合前期构建的转录组数据挖掘葫芦素E代谢相关关键基因，共筛选得到9个参与葫芦素E生物合成的候选基因，与其他葫芦科作物同源基因作同源性比对及系统进化树构建，初步推测细胞色素P450酶基因家族、氧化角鲨烯环化酶（OSC）基因、转录因子ClBt基因和乙酰转移酶（ACT）基因上调表达与西瓜果实中葫芦素E积累相关，Cla007078、ACT和OSC基因为西瓜葫芦素E合成代谢相关候选基因。研究为进一步探究西瓜葫芦素E积累相关基因功能提供参考。更多还原

【Abstract】 The contents of cucurbitacin E in watermelon fruits, leaves, and stems at different developmental stages were determined by high performance liquid chromatography(HPLC), the key genes involved in cucurbitacin E biosynthesis in Cucurbitaceae database were identified, and the key genes related to cucurbitacin E metabolism were mined by combining with the transcriptome data created in the previous study to explore the mechanism of cucurbitacin E accumulation in watermelon(Citrullus lanatus) fruits at different developmental stages. Nine candidate genes involved in cucurbitacin E biosynthesis were screened and performed homology comparison with the homologous genes of other Cucurbitaceae crops, and a phylogenetic tree was constructed. The initial hypothesis was that the up-regulated expression of the cytochrome P450 enzyme gene family, oxysqualene cyclase(OSC) gene, transcription factor ClBt, and acetyltransferase(ACT) gene was related to the accumulation of cucurbitacin E in watermelon fruits. All of the above results indicated that the candidate genes Cla007078, ACT, and OSC were postulated to be involved in the synthesis and metabolism of cucurbitacin E in watermelon. These results could serve as a reference for future studies on the roles of watermelon cucurbitacin E-related genes.更多还原